2cjk
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2cjk.jpg|left|200px]] | + | [[Image:2cjk.jpg|left|200px]] |
| - | + | ||
| - | '''STRUCTURE OF THE RNA BINDING DOMAIN OF HRP1 IN COMPLEX WITH RNA''' | + | {{Structure |
| + | |PDB= 2cjk |SIZE=350|CAPTION= <scene name='initialview01'>2cjk</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF THE RNA BINDING DOMAIN OF HRP1 IN COMPLEX WITH RNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2CJK is a [ | + | 2CJK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJK OCA]. |
==Reference== | ==Reference== | ||
| - | Grabbing the message: structural basis of mRNA 3'UTR recognition by Hrp1., Perez-Canadillas JM, EMBO J. 2006 Jul 12;25(13):3167-78. Epub 2006 Jun 22. PMID:[http:// | + | Grabbing the message: structural basis of mRNA 3'UTR recognition by Hrp1., Perez-Canadillas JM, EMBO J. 2006 Jul 12;25(13):3167-78. Epub 2006 Jun 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16794580 16794580] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: rna-binding protein]] | [[Category: rna-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:16:13 2008'' |
Revision as of 14:16, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE RNA BINDING DOMAIN OF HRP1 IN COMPLEX WITH RNA
Overview
The recognition of specific signals encoded within the 3'-untranslated region of the newly transcribed mRNA triggers the assembly of a multiprotein machine that modifies its 3'-end. Hrp1 recognises one of such signals, the so-called polyadenylation enhancement element (PEE), promoting the recruitment of other polyadenylation factors in yeast. The molecular bases of this interaction are revealed here by the solution structure of a complex between Hrp1 and an oligonucleotide mimicking the PEE. Six consecutive bases (AUAUAU) are specifically recognised by two RNA-binding domains arranged in tandem. Both protein and RNA undergo significant conformational changes upon complex formation with a concomitant large surface burial of RNA bases. Key aspects of RNA specificity can be explained by the presence of intermolecular aromatic-aromatic contacts and hydrogen bonds. Altogether, the Hrp1-PEE structure represents one of the first steps towards understanding of the assembly of the cleavage and polyadenylation machinery at the atomic level.
About this Structure
2CJK is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Grabbing the message: structural basis of mRNA 3'UTR recognition by Hrp1., Perez-Canadillas JM, EMBO J. 2006 Jul 12;25(13):3167-78. Epub 2006 Jun 22. PMID:16794580
Page seeded by OCA on Thu Mar 20 16:16:13 2008
