2czt

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[[Image:2czt.gif|left|200px]]<br /><applet load="2czt" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2czt.gif|left|200px]]
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caption="2czt, resolution 2.00&Aring;" />
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'''lipocalin-type prostaglandin D synthase'''<br />
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{{Structure
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|PDB= 2czt |SIZE=350|CAPTION= <scene name='initialview01'>2czt</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/Prostaglandin-D_synthase Prostaglandin-D synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.2 5.3.99.2]
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|GENE=
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}}
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'''lipocalin-type prostaglandin D synthase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2CZT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Prostaglandin-D_synthase Prostaglandin-D synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.2 5.3.99.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZT OCA].
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2CZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZT OCA].
==Reference==
==Reference==
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Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12761195 12761195]
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Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12761195 12761195]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Prostaglandin-D synthase]]
[[Category: Prostaglandin-D synthase]]
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[[Category: riken structural genomics/proteomics initiative]]
[[Category: riken structural genomics/proteomics initiative]]
[[Category: rsgi]]
[[Category: rsgi]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:53:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:21:37 2008''

Revision as of 14:21, 20 March 2008

Image:2czt.gif


PDB ID 2czt

Drag the structure with the mouse to rotate
, resolution 2.00Å
Activity: Prostaglandin-D synthase, with EC number 5.3.99.2
Coordinates: save as pdb, mmCIF, xml



lipocalin-type prostaglandin D synthase


Overview

Lipocalin-type prostaglandin D synthase is the key enzyme for the production of prostaglandin D(2), a potent endogenous somnogen, in the brain. We cloned, produced, and crystallized the native enzyme and selenomethionyl Cys(65)Ala mutants of the recombinant mouse protein by the hanging drop vapor-diffusion method with both malonate and citrate as precipitants. The native crystals obtained with malonate belong to orthorhombic space group P2(1)2(1)2(1) with lattice constants a = 46.2, b = 66.8, and c = 105.3 A. The selenomethionyl crystals obtained with citrate belong to orthorhombic space group C222(1) with lattice constants a = 45.5, b = 66.8, and c = 104.5 A. The native crystals diffracted beyond 2.1 A resolution.

About this Structure

2CZT is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:12761195

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