2d1g

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[[Image:2d1g.gif|left|200px]]<br /><applet load="2d1g" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2d1g.gif|left|200px]]
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caption="2d1g, resolution 1.750&Aring;" />
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'''Structure of Francisella tularensis Acid Phosphatase A (AcpA) bound to orthovanadate'''<br />
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{{Structure
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|PDB= 2d1g |SIZE=350|CAPTION= <scene name='initialview01'>2d1g</scene>, resolution 1.750&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>, <scene name='pdbligand=DVT:DECAVANADATE'>DVT</scene>, <scene name='pdbligand=ETE:2-{2-[2-2-(METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>ETE</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene> and <scene name='pdbligand=ETX:2-ETHOXYETHANOL'>ETX</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2]
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|GENE= acpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264 Francisella tularensis subsp. novicida])
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}}
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'''Structure of Francisella tularensis Acid Phosphatase A (AcpA) bound to orthovanadate'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2D1G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida Francisella tularensis subsp. novicida] with <scene name='pdbligand=VO4:'>VO4</scene>, <scene name='pdbligand=UNX:'>UNX</scene>, <scene name='pdbligand=DVT:'>DVT</scene>, <scene name='pdbligand=ETE:'>ETE</scene>, <scene name='pdbligand=PGE:'>PGE</scene> and <scene name='pdbligand=ETX:'>ETX</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1G OCA].
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2D1G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida Francisella tularensis subsp. novicida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1G OCA].
==Reference==
==Reference==
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Structure of Francisella tularensis AcpA: prototype of a unique superfamily of acid phosphatases and phospholipases C., Felts RL, Reilly TJ, Tanner JJ, J Biol Chem. 2006 Oct 6;281(40):30289-98. Epub 2006 Aug 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16899453 16899453]
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Structure of Francisella tularensis AcpA: prototype of a unique superfamily of acid phosphatases and phospholipases C., Felts RL, Reilly TJ, Tanner JJ, J Biol Chem. 2006 Oct 6;281(40):30289-98. Epub 2006 Aug 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16899453 16899453]
[[Category: Acid phosphatase]]
[[Category: Acid phosphatase]]
[[Category: Francisella tularensis subsp. novicida]]
[[Category: Francisella tularensis subsp. novicida]]
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[[Category: vanadate]]
[[Category: vanadate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:54:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:22:08 2008''

Revision as of 14:22, 20 March 2008

Image:2d1g.gif


PDB ID 2d1g

Drag the structure with the mouse to rotate
, resolution 1.750Å
Ligands: , , , , and
Gene: acpA (Francisella tularensis subsp. novicida)
Activity: Acid phosphatase, with EC number 3.1.3.2
Coordinates: save as pdb, mmCIF, xml



Structure of Francisella tularensis Acid Phosphatase A (AcpA) bound to orthovanadate


Overview

AcpA is a respiratory burst-inhibiting acid phosphatase from the Centers for Disease Control and Prevention Category A bioterrorism agent Francisella tularensis and prototype of a superfamily of acid phosphatases and phospholipases C. We report the 1.75-A resolution crystal structure of AcpA complexed with the inhibitor orthovanadate, which is the first structure of any F. tularensis protein and the first for any member of this superfamily. The core domain is a twisted 8-stranded beta-sheet flanked by three alpha-helices on either side, with the active site located above the carboxyl-terminal edge of the beta-sheet. This architecture is unique among acid phosphatases and resembles that of alkaline phosphatase. Unexpectedly, the active site features a serine nucleophile and metal ion with octahedral coordination. Structure-based sequence analysis of the AcpA superfamily predicts that the hydroxyl nucleophile and metal center are also present in AcpA-like phospholipases C. These results imply a phospholipase C catalytic mechanism that is radically different from that of zinc metallophospholipases.

About this Structure

2D1G is a Single protein structure of sequence from Francisella tularensis subsp. novicida. Full crystallographic information is available from OCA.

Reference

Structure of Francisella tularensis AcpA: prototype of a unique superfamily of acid phosphatases and phospholipases C., Felts RL, Reilly TJ, Tanner JJ, J Biol Chem. 2006 Oct 6;281(40):30289-98. Epub 2006 Aug 9. PMID:16899453

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