2d1k
From Proteopedia
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| - | [[Image:2d1k.gif|left|200px]] | + | [[Image:2d1k.gif|left|200px]] |
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| - | '''Ternary complex of the WH2 domain of mim with actin-dnase I''' | + | {{Structure |
| + | |PDB= 2d1k |SIZE=350|CAPTION= <scene name='initialview01'>2d1k</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Ternary complex of the WH2 domain of mim with actin-dnase I''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2D1K is a [ | + | 2D1K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1K OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the actin-binding function of missing-in-metastasis., Lee SH, Kerff F, Chereau D, Ferron F, Klug A, Dominguez R, Structure. 2007 Feb;15(2):145-55. PMID:[http:// | + | Structural basis for the actin-binding function of missing-in-metastasis., Lee SH, Kerff F, Chereau D, Ferron F, Klug A, Dominguez R, Structure. 2007 Feb;15(2):145-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17292833 17292833] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Deoxyribonuclease I]] | [[Category: Deoxyribonuclease I]] | ||
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[[Category: wip]] | [[Category: wip]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:22:10 2008'' |
Revision as of 14:22, 20 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Activity: | Deoxyribonuclease I, with EC number 3.1.21.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Ternary complex of the WH2 domain of mim with actin-dnase I
Overview
The adaptor protein missing-in-metastasis (MIM) contains independent F- and G-actin binding domains, consisting, respectively, of an N-terminal 250 aa IRSp53/MIM homology domain (IMD) and a C-terminal WASP-homology domain 2 (WH2). We determined the crystal structures of MIM's IMD and that of its WH2 bound to actin. The IMD forms a dimer, with each subunit folded as an antiparallel three-helix bundle. This fold is related to that of the BAR domain. Like the BAR domain, the IMD has been implicated in membrane binding. Yet, comparison of the structures reveals that the membrane binding surfaces of the two domains have opposite curvatures, which may determine the type of curvature of the interacting membrane. The WH2 of MIM is longer than the prototypical WH2, interacting with all four subdomains of actin. We characterize a similar WH2 at the C terminus of IRSp53 and propose that in these two proteins WH2 performs a scaffolding function.
About this Structure
2D1K is a Protein complex structure of sequences from Bos taurus and Oryctolagus cuniculus. Full crystallographic information is available from OCA.
Reference
Structural basis for the actin-binding function of missing-in-metastasis., Lee SH, Kerff F, Chereau D, Ferron F, Klug A, Dominguez R, Structure. 2007 Feb;15(2):145-55. PMID:17292833
Page seeded by OCA on Thu Mar 20 16:22:10 2008
Categories: Bos taurus | Deoxyribonuclease I | Oryctolagus cuniculus | Protein complex | Chereau, D. | Dominguez, R. | Kerff, F. | ATP | CA | MG | Actin | Dnase i | Wasp | Wh2 | Wip
