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2d36
From Proteopedia
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| - | [[Image:2d36.gif|left|200px]] | + | [[Image:2d36.gif|left|200px]] |
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| - | '''The Crystal Structure of Flavin Reductase HpaC''' | + | {{Structure |
| + | |PDB= 2d36 |SIZE=350|CAPTION= <scene name='initialview01'>2d36</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FMN:FLAVIN MONONUCLEOTIDE'>FMN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''The Crystal Structure of Flavin Reductase HpaC''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2D36 is a [ | + | 2D36 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii_str._7 Sulfolobus tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D36 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of the short-chain flavin reductase HpaC from Sulfolobus tokodaii strain 7 in its three states: NAD(P)(+)(-)free, NAD(+)(-)bound, and NADP(+)(-)bound., Okai M, Kudo N, Lee WC, Kamo M, Nagata K, Tanokura M, Biochemistry. 2006 Apr 25;45(16):5103-10. PMID:[http:// | + | Crystal structures of the short-chain flavin reductase HpaC from Sulfolobus tokodaii strain 7 in its three states: NAD(P)(+)(-)free, NAD(+)(-)bound, and NADP(+)(-)bound., Okai M, Kudo N, Lee WC, Kamo M, Nagata K, Tanokura M, Biochemistry. 2006 Apr 25;45(16):5103-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16618099 16618099] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus tokodaii str. 7]] | [[Category: Sulfolobus tokodaii str. 7]] | ||
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[[Category: flavin reductase]] | [[Category: flavin reductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:22:46 2008'' |
Revision as of 14:22, 20 March 2008
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| , resolution 2.30Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Crystal Structure of Flavin Reductase HpaC
Overview
4-Hydroxyphenylacetate (4-HPA) is oxidized as an energy source by two component enzymes, the large component (HpaB) and the small component (HpaC). HpaB is a 4-HPA monooxygenase that utilizes FADH(2) supplied by a flavin reductase HpaC. We determined the crystal structure of HpaC (ST0723) from the aerobic thermoacidophilic crenarchaeon Sulfolobus tokodaii strain 7 in its three states [NAD(P)(+)-free, NAD(+)-bound, and NADP(+)-bound]. HpaC exists as a homodimer, and each monomer was found to contain an FMN. HpaC preferred FMN to FAD because there was not enough space to accommodate the AMP moiety of FAD in its flavin-binding site. The most striking difference between the NAD(P)(+)-free and the NAD(+)/NADP(+)-bound structures was observed in the N-terminal helix. The N-terminal helices in the NAD(+)/NADP(+)-bound structures rotated ca. 20 degrees relative to the NAD(P)(+)-free structure. The bound NAD(+) has a compact folded conformation with nearly parallel stacking rings of nicotinamide and adenine. The nicotinamide of NAD(+) stacked the isoalloxazine ring of FMN so that NADH could directly transfer hydride. The bound NADP(+) also had a compact conformation but was bound in a reverse direction, which was not suitable for hydride transfer.
About this Structure
2D36 is a Single protein structure of sequence from Sulfolobus tokodaii str. 7. Full crystallographic information is available from OCA.
Reference
Crystal structures of the short-chain flavin reductase HpaC from Sulfolobus tokodaii strain 7 in its three states: NAD(P)(+)(-)free, NAD(+)(-)bound, and NADP(+)(-)bound., Okai M, Kudo N, Lee WC, Kamo M, Nagata K, Tanokura M, Biochemistry. 2006 Apr 25;45(16):5103-10. PMID:16618099
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