2d4j

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[[Image:2d4j.gif|left|200px]]<br /><applet load="2d4j" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2d4j.gif|left|200px]]
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caption="2d4j, resolution 1.16&Aring;" />
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'''Transformed monoclinic crystal of hen egg-white lysozyme from a heavy water solution'''<br />
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{{Structure
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|PDB= 2d4j |SIZE=350|CAPTION= <scene name='initialview01'>2d4j</scene>, resolution 1.16&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene> and <scene name='pdbligand=DOD:DEUTERATED WATER'>DOD</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]
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|GENE=
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}}
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'''Transformed monoclinic crystal of hen egg-white lysozyme from a heavy water solution'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2D4J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=NO3:'>NO3</scene> and <scene name='pdbligand=DOD:'>DOD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D4J OCA].
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2D4J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D4J OCA].
==Reference==
==Reference==
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Structural phase transition of monoclinic crystals of hen egg-white lysozyme., Harata K, Akiba T, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):375-82. Epub 2006, Mar 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16552138 16552138]
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Structural phase transition of monoclinic crystals of hen egg-white lysozyme., Harata K, Akiba T, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):375-82. Epub 2006, Mar 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16552138 16552138]
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
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[[Category: tls analysis]]
[[Category: tls analysis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:55:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:23:15 2008''

Revision as of 14:23, 20 March 2008

Image:2d4j.gif


PDB ID 2d4j

Drag the structure with the mouse to rotate
, resolution 1.16Å
Ligands: and
Activity: Lysozyme, with EC number 3.2.1.17
Coordinates: save as pdb, mmCIF, xml



Transformed monoclinic crystal of hen egg-white lysozyme from a heavy water solution


Overview

Two monoclinic crystals (space group P2(1)) of hen egg-white lysozyme, a type I crystal grown at room temperature in a D2O solution with pD 4.5 containing 2%(w/v) sodium nitrate and a type II crystal grown at 313 K in a 10%(w/v) sodium chloride solution with pH 7.6, were each transformed into another monoclinic crystal with the same space group by dehydration-induced phase transition. Changes in X-ray diffraction were recorded to monitor the progress of the crystal transformation, which started with the appearance of diffuse streaks. In both crystals, the intensity of h + l odd reflections gradually weakened and finally disappeared on completion of the transformation. X-ray diffraction in the intermediate state indicated the presence of lattices of both the native and transformed crystals. In the native type I crystal, two alternate conformations were observed in the main chain of the region Gly71-Asn74. One conformer bound a sodium ion which was replaced with a water molecule in the other conformer. In the transformed crystal, the sodium ion was removed and the main-chain conformation of this region was converted to that of the water-bound form. The transformed crystal diffracted to a higher resolution than the native crystal, while the peak width of the diffraction spots increased. Analysis of the thermal motion of protein molecules using the TLS model has shown that the enhancement of the diffraction power in the transformed crystal is mainly ascribable to the suppression of rigid-body motion owing to an increase in intermolecular contacts as a result of the loss of bulk solvent.

About this Structure

2D4J is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Structural phase transition of monoclinic crystals of hen egg-white lysozyme., Harata K, Akiba T, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):375-82. Epub 2006, Mar 18. PMID:16552138

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