2d5x

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[[Image:2d5x.gif|left|200px]]<br /><applet load="2d5x" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2d5x.gif|left|200px]]
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caption="2d5x, resolution 1.45&Aring;" />
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'''Crystal structure of carbonmonoxy horse hemoglobin complexed with L35'''<br />
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{{Structure
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|PDB= 2d5x |SIZE=350|CAPTION= <scene name='initialview01'>2d5x</scene>, resolution 1.45&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene> and <scene name='pdbligand=L35:2-[4-({[(3,5-DICHLOROPHENYL)AMINO]CARBONYL}AMINO)PHENOXY]-2-METHYLPROPANOIC ACID'>L35</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure of carbonmonoxy horse hemoglobin complexed with L35'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2D5X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus] with <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=CMO:'>CMO</scene> and <scene name='pdbligand=L35:'>L35</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5X OCA].
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2D5X is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5X OCA].
==Reference==
==Reference==
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R-state haemoglobin with low oxygen affinity: crystal structures of deoxy human and carbonmonoxy horse haemoglobin bound to the effector molecule L35., Yokoyama T, Neya S, Tsuneshige A, Yonetani T, Park SY, Tame JR, J Mol Biol. 2006 Feb 24;356(3):790-801. Epub 2005 Dec 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16403522 16403522]
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R-state haemoglobin with low oxygen affinity: crystal structures of deoxy human and carbonmonoxy horse haemoglobin bound to the effector molecule L35., Yokoyama T, Neya S, Tsuneshige A, Yonetani T, Park SY, Tame JR, J Mol Biol. 2006 Feb 24;356(3):790-801. Epub 2005 Dec 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16403522 16403522]
[[Category: Equus caballus]]
[[Category: Equus caballus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: l35]]
[[Category: l35]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:55:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:23:38 2008''

Revision as of 14:23, 20 March 2008

Image:2d5x.gif


PDB ID 2d5x

Drag the structure with the mouse to rotate
, resolution 1.45Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



Crystal structure of carbonmonoxy horse hemoglobin complexed with L35


Overview

Although detailed crystal structures of haemoglobin (Hb) provide a clear understanding of the basic allosteric mechanism of the protein, and how this in turn controls oxygen affinity, recent experiments with artificial effector molecules have shown a far greater control of oxygen binding than with natural heterotropic effectors. Contrary to the established text-book view, these non-physiological compounds are able to reduce oxygen affinity very strongly without switching the protein to the T (tense) state. In an earlier paper we showed that bezafibrate (BZF) binds to a surface pocket on the alpha subunits of R state Hb, strongly reducing the oxygen affinity of this protein conformation. Here we report the crystallisation of Hb with L35, a related compound, and show that this binds to the central cavity of both R and T state Hb. The mechanism by which L35 reduces oxygen affinity is discussed, in relation to spectroscopic studies of effector binding.

About this Structure

2D5X is a Protein complex structure of sequences from Equus caballus. Full crystallographic information is available from OCA.

Reference

R-state haemoglobin with low oxygen affinity: crystal structures of deoxy human and carbonmonoxy horse haemoglobin bound to the effector molecule L35., Yokoyama T, Neya S, Tsuneshige A, Yonetani T, Park SY, Tame JR, J Mol Biol. 2006 Feb 24;356(3):790-801. Epub 2005 Dec 21. PMID:16403522

Page seeded by OCA on Thu Mar 20 16:23:38 2008

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