2d5v
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2d5v.gif|left|200px]] | + | [[Image:2d5v.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of HNF-6alpha DNA-binding domain in complex with the TTR promoter''' | + | {{Structure |
| + | |PDB= 2d5v |SIZE=350|CAPTION= <scene name='initialview01'>2d5v</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of HNF-6alpha DNA-binding domain in complex with the TTR promoter''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2D5V is a [ | + | 2D5V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5V OCA]. |
==Reference== | ==Reference== | ||
| - | DNA recognition mechanism of the ONECUT homeodomain of transcription factor HNF-6., Iyaguchi D, Yao M, Watanabe N, Nishihira J, Tanaka I, Structure. 2007 Jan;15(1):75-83. PMID:[http:// | + | DNA recognition mechanism of the ONECUT homeodomain of transcription factor HNF-6., Iyaguchi D, Yao M, Watanabe N, Nishihira J, Tanaka I, Structure. 2007 Jan;15(1):75-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17223534 17223534] |
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:23:38 2008'' |
Revision as of 14:23, 20 March 2008
| |||||||
| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of HNF-6alpha DNA-binding domain in complex with the TTR promoter
Overview
Hepatocyte nuclear factor-6 (HNF-6), a liver-enriched transcription factor, controls the development of various tissues, such as the pancreas and liver, and regulates the expression of several hepatic genes. This protein belongs to the ONECUT class of homeodomain proteins and contains a bipartite DNA-binding domain composed of a single cut domain and a characteristic homeodomain. This transcription factor has two distinct modes of DNA binding and transcriptional activation that use different coactivators depending on the target gene. The crystal structure of the bipartite DNA-binding domain of HNF-6alpha complexed with the HNF-6-binding site of the TTR promoter revealed the DNA recognition mechanism of this protein. Comparing our structure with the DNA-free structure of HNF-6 or the structure of Oct-1, we discuss characteristic features associated with DNA binding and the structural basis for the dual mode of action of this protein, and we suggest a strategy for variability of transcriptional activation of the target gene.
About this Structure
2D5V is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
DNA recognition mechanism of the ONECUT homeodomain of transcription factor HNF-6., Iyaguchi D, Yao M, Watanabe N, Nishihira J, Tanaka I, Structure. 2007 Jan;15(1):75-83. PMID:17223534
Page seeded by OCA on Thu Mar 20 16:23:38 2008
