2csa
From Proteopedia
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| - | + | ==Structure of the M3 Muscarinic Acetylcholine Receptor Basolateral Sorting Signal== | |
| - | + | <StructureSection load='2csa' size='340' side='right' caption='[[2csa]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2csa]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CSA FirstGlance]. <br> | |
| - | ==Disease== | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2csa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2csa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2csa RCSB], [http://www.ebi.ac.uk/pdbsum/2csa PDBsum]</span></td></tr> |
| - | [[http://www.uniprot.org/uniprot/ACM3_HUMAN ACM3_HUMAN]] Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS) [MIM:[http://omim.org/entry/100100 100100]]. EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities.<ref>PMID:22077972</ref> | + | </table> |
| - | + | == Disease == | |
| - | ==Function== | + | [[http://www.uniprot.org/uniprot/ACM3_HUMAN ACM3_HUMAN]] Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS) [MIM:[http://omim.org/entry/100100 100100]]. EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities.<ref>PMID:22077972</ref> |
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/ACM3_HUMAN ACM3_HUMAN]] The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. | [[http://www.uniprot.org/uniprot/ACM3_HUMAN ACM3_HUMAN]] The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Muscarinic acetylcholine receptors comprise a family of G-protein-coupled receptors that display differential localization in polarized epithelial cells. We identify a seven-residue sequence, Ala(275)-Val(281), in the third intracellular loop of the M(3) muscarinic receptor that mediates dominant, position-independent basolateral targeting in Madin-Darby canine kidney cells. Mutational analyses identify Glu(276), Phe(280), and Val(281) as critical residues within this sorting motif. Phe(280) and Val(281) comprise a novel dihydrophobic sorting signal as mutations of either residue singly or together with leucine do not disrupt basolateral targeting. Conversely, Glu(276) is required and cannot be substituted with alanine or aspartic acid. A 19-amino acid peptide representing the M(3) sorting signal and surrounding sequence was analyzed via two-dimensional nuclear magnetic resonance spectroscopy. Solution structures show that Glu(276) resides in a type IV beta-turn and the dihydrophobic sequence Phe(280)Val(281) adopts either a type I or IV beta-turn. | ||
| - | + | Identification and structural determination of the M(3) muscarinic acetylcholine receptor basolateral sorting signal.,Iverson HA, Fox D 3rd, Nadler LS, Klevit RE, Nathanson NM J Biol Chem. 2005 Jul 1;280(26):24568-75. Epub 2005 May 2. PMID:15870063<ref>PMID:15870063</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | <references | + | </div> |
| - | [[Category: Fox, D | + | == References == |
| - | [[Category: Iverson, H A | + | <references/> |
| - | [[Category: Klevit, R E | + | __TOC__ |
| - | [[Category: Nadler, L S | + | </StructureSection> |
| - | [[Category: Nathanson, N M | + | [[Category: Fox, D]] |
| + | [[Category: Iverson, H A]] | ||
| + | [[Category: Klevit, R E]] | ||
| + | [[Category: Nadler, L S]] | ||
| + | [[Category: Nathanson, N M]] | ||
[[Category: Basolateral sorting-signal blss beta-turn]] | [[Category: Basolateral sorting-signal blss beta-turn]] | ||
[[Category: Signaling protein-membrane protein complex]] | [[Category: Signaling protein-membrane protein complex]] | ||
Revision as of 11:33, 18 December 2014
Structure of the M3 Muscarinic Acetylcholine Receptor Basolateral Sorting Signal
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