2dbt
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2dbt.gif|left|200px]]<br /><applet load="2dbt" size="350" color="white" frame="true" align="right" | + | [[Image:2dbt.gif|left|200px]]<br /><applet load="2dbt" size="350" color="white" frame="true" align="right" caption="2dbt, resolution 3.14Å" /> |
| - | caption="2dbt, resolution 3.14Å" /> | + | |
'''Crystal structure of chitinase C from Streptomyces griseus HUT6037'''<br /> | '''Crystal structure of chitinase C from Streptomyces griseus HUT6037'''<br /> | ||
| Line 7: | Line 6: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2DBT is a [ | + | 2DBT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_chryseus Streptomyces chryseus] with <scene name='pdbligand=EPE:'>EPE</scene> as [[ligand]]. Active as [http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DBT OCA]. |
==Reference== | ==Reference== | ||
| - | Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037., Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T, J Mol Biol. 2006 Apr 28;358(2):472-84. Epub 2006 Feb 21. PMID:[http:// | + | Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037., Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T, J Mol Biol. 2006 Apr 28;358(2):472-84. Epub 2006 Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16516924 16516924] |
[[Category: Chitinase]] | [[Category: Chitinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 19: | ||
[[Category: family 19 chitinase]] | [[Category: family 19 chitinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Mar 18 13:26:31 2008'' |
Revision as of 11:26, 18 March 2008
|
Crystal structure of chitinase C from Streptomyces griseus HUT6037
Overview
Chitinase C (ChiC) from Streptomyces griseus HUT6037 was the first glycoside hydrolase family 19 chitinase that was found in an organism other than higher plants. An N-terminal chitin-binding domain and a C-terminal catalytic domain connected by a linker peptide constitute ChiC. We determined the crystal structure of full-length ChiC, which is the only representative of the two-domain chitinases in the family. The catalytic domain has an alpha-helix-rich fold with a deep cleft containing a catalytic site, and lacks three loops on the domain surface compared with the catalytic domain of plant chitinases. The chitin-binding domain is an all-beta protein with two tryptophan residues (Trp59 and Trp60) aligned on the surface. We suggest the binding mechanism of tri-N-acetylchitotriose onto the chitin-binding domain on the basis of molecular dynamics (MD) simulations. In this mechanism, the ligand molecule binds well on the surface-exposed binding site through two stacking interactions and two hydrogen bonds and only Trp59 and Trp60 are involved in the binding. Furthermore, the flexibility of the Trp60 side-chain, which may be involved in adjusting the binding surface to fit the surface of crystalline chitin by the rotation of chi2 angle, is shown.
About this Structure
2DBT is a Single protein structure of sequence from Streptomyces chryseus with as ligand. Active as Chitinase, with EC number 3.2.1.14 Full crystallographic information is available from OCA.
Reference
Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037., Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T, J Mol Biol. 2006 Apr 28;358(2):472-84. Epub 2006 Feb 21. PMID:16516924
Page seeded by OCA on Tue Mar 18 13:26:31 2008
