2dga
From Proteopedia
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| - | [[Image:2dga.gif|left|200px]] | + | [[Image:2dga.gif|left|200px]] |
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| - | '''Crystal structure of hexameric beta-glucosidase in wheat''' | + | {{Structure |
| + | |PDB= 2dga |SIZE=350|CAPTION= <scene name='initialview01'>2dga</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] | ||
| + | |GENE= Taglu1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4565 Triticum aestivum]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of hexameric beta-glucosidase in wheat''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DGA is a [ | + | 2DGA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DGA OCA]. |
==Reference== | ==Reference== | ||
| - | Molecular and structural characterization of hexameric beta-D-glucosidases in wheat and rye., Sue M, Yamazaki K, Yajima S, Nomura T, Matsukawa T, Iwamura H, Miyamoto T, Plant Physiol. 2006 Aug;141(4):1237-47. Epub 2006 Jun 2. PMID:[http:// | + | Molecular and structural characterization of hexameric beta-D-glucosidases in wheat and rye., Sue M, Yamazaki K, Yajima S, Nomura T, Matsukawa T, Iwamura H, Miyamoto T, Plant Physiol. 2006 Aug;141(4):1237-47. Epub 2006 Jun 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16751439 16751439] |
[[Category: Beta-glucosidase]] | [[Category: Beta-glucosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: alpha/beta barrel]] | [[Category: alpha/beta barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:26:53 2008'' |
Revision as of 14:26, 20 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | Taglu1b (Triticum aestivum) | ||||||
| Activity: | Beta-glucosidase, with EC number 3.2.1.21 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of hexameric beta-glucosidase in wheat
Overview
The wheat (Triticum aestivum) and rye (Secale cereale) beta-D-glucosidases hydrolyze hydroxamic acid-glucose conjugates, exist as different types of isozyme, and function as oligomers. In this study, three cDNAs encoding beta-D-glucosidases (TaGlu1a, TaGlu1b, and TaGlu1c) were isolated from young wheat shoots. Although the TaGlu1s share very high sequence homology, the mRNA level of Taglu1c was much lower than the other two genes in 48- and 96-h-old wheat shoots. The expression ratio of each gene was different between two wheat cultivars. Recombinant TaGlu1b expressed in Escherichia coli was electrophoretically distinct fromTaGlu1a and TaGlu1c. Furthermore, coexpression of TaGlu1a and TaGlu1b gave seven bands on a native-PAGE gel, indicating the formation of both homo- and heterohexamers. One distinctive property of the wheat and rye glucosidases is that they function as hexamers but lose activity when dissociated into smaller oligomers or monomers. The crystal structure of hexameric TaGlu1b was determined at a resolution of 1.8 A. The N-terminal region was located at the dimer-dimer interface and plays a crucial role in hexamer formation. Mutational analyses revealed that the aromatic side chain at position 378, which is located at the entrance to the catalytic center, plays an important role in substrate binding. Additionally, serine-464 and leucine-465 of TaGlu1a were shown to be critical in the relative specificity for DIMBOA-glucose (2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one) over DIBOA-glucose (7-demethoxy-DIMBOA-glucose).
About this Structure
2DGA is a Single protein structure of sequence from Triticum aestivum. Full crystallographic information is available from OCA.
Reference
Molecular and structural characterization of hexameric beta-D-glucosidases in wheat and rye., Sue M, Yamazaki K, Yajima S, Nomura T, Matsukawa T, Iwamura H, Miyamoto T, Plant Physiol. 2006 Aug;141(4):1237-47. Epub 2006 Jun 2. PMID:16751439
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