3is2

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{{STRUCTURE_3is2| PDB=3is2 | SCENE= }}
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==2.3 Angstrom Crystal Structure of a Cys71 Sulfenic Acid form of Vivid==
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===2.3 Angstrom Crystal Structure of a Cys71 Sulfenic Acid form of Vivid===
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<StructureSection load='3is2' size='340' side='right' caption='[[3is2]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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{{ABSTRACT_PUBMED_19712683}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3is2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IS2 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2pd7|2pd7]], [[2pd8|2pd8]], [[2pdr|2pdr]], [[2pdt|2pdt]], [[3d72|3d72]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">G17A4.050, Vivid, vvd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Neurospora crassa])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3is2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3is2 RCSB], [http://www.ebi.ac.uk/pdbsum/3is2 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/is/3is2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The PAS-LOV domain is a signal-transducing component found in a large variety of proteins that is responsible for sensing different stimuli such as light, oxygen, and voltage. The LOV protein VVD regulates blue light responses in the filamentous fungi Neurospora crassa. Using photocoupled, time-resolved small-angle X-ray scattering, we extract the solution protein structure in both dark-adapted and light-activated states. Two distinct dark-adapted conformations are detected in the wild-type protein: a compact structure that corresponds to the crystal structure of the dark-state monomer as well as an extended structure that is well modeled by introducing conformational disorder at the N-terminus of the protein. These conformations are accentuated in carefully selected variants, in which a key residue for propagating structural transitions, Cys71, has been mutated or oxidized. Despite different dark-state conformations, all proteins form a common dimer in response to illumination. Taken together, these data support a reaction scheme that describes the mechanism for light-induced dimerization of VVD. Envelope reconstructions of the transient light-state dimer reveal structures that are best described by a parallel arrangement of subunits that have significantly changed conformation compared to the crystal structure.
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==About this Structure==
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Illuminating solution responses of a LOV domain protein with photocoupled small-angle X-ray scattering.,Lamb JS, Zoltowski BD, Pabit SA, Li L, Crane BR, Pollack L J Mol Biol. 2009 Nov 6;393(4):909-19. Epub 2009 Aug 25. PMID:19712683<ref>PMID:19712683</ref>
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[[3is2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IS2 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:019712683</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Neurospora crassa]]
[[Category: Neurospora crassa]]
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[[Category: Crane, B R.]]
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[[Category: Crane, B R]]
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[[Category: Lamb, J S.]]
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[[Category: Lamb, J S]]
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[[Category: Li, L.]]
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[[Category: Li, L]]
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[[Category: Pabit, S A.]]
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[[Category: Pabit, S A]]
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[[Category: Pollack, L.]]
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[[Category: Pollack, L]]
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[[Category: Zoltowski, B D.]]
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[[Category: Zoltowski, B D]]
[[Category: Circadian clock]]
[[Category: Circadian clock]]
[[Category: Flavin]]
[[Category: Flavin]]

Revision as of 15:45, 18 December 2014

2.3 Angstrom Crystal Structure of a Cys71 Sulfenic Acid form of Vivid

3is2, resolution 2.30Å

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