3iso

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{{STRUCTURE_3iso| PDB=3iso | SCENE= }}
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==Crystal structure of 26 kDa GST of Clonorchis sinensis in P3221 symmetry==
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===Crystal structure of 26 kDa GST of Clonorchis sinensis in P3221 symmetry===
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<StructureSection load='3iso' size='340' side='right' caption='[[3iso]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20347989}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3iso]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clonorchis_sinensis Clonorchis sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ISO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ISO FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iso OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iso RCSB], [http://www.ebi.ac.uk/pdbsum/3iso PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/is/3iso_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glutathione S-transferases (GSTs) are multifunctional enzymes that are used as fusion tags on recombinant proteins in mammalian and Escherichia coli expression systems. We recently found that the Schistosoma japonicum GST (SjGST) displays weak Ni(2+) ion binding affinity. Glu26 and His79 were assumed to be its Ni(2+) binding sites based on the structure of the 26-kDa Clonorchis sinensis GST. To enhance SjGST Ni(2+) binding affinity, Glu26 was mutated to His. SjGST-E26H was expressed and purified at a high concentration of imidazole to a higher purity than wild type SjGST. In addition, human biotin protein ligase fused to SjGST-E26H was purified with a immobilized Ni affinity column.
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==About this Structure==
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A histidine substitution confers metal binding affinity to a Schistosoma japonicum Glutathione S-transferase.,Han YH, Seo HA, Kim GH, Lee CK, Kang YK, Ryu KH, Chung YJ Protein Expr Purif. 2010 Sep;73(1):74-7. Epub 2010 Mar 27. PMID:20347989<ref>PMID:20347989</ref>
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[[3iso]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clonorchis_sinensis Clonorchis sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ISO OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020347989</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Clonorchis sinensis]]
[[Category: Clonorchis sinensis]]
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[[Category: Chung, Y J.]]
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[[Category: Chung, Y J]]
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[[Category: Han, Y H.]]
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[[Category: Han, Y H]]
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[[Category: Kim, G H.]]
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[[Category: Kim, G H]]
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[[Category: Seo, H A.]]
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[[Category: Seo, H A]]
[[Category: Gst]]
[[Category: Gst]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 16:24, 18 December 2014

Crystal structure of 26 kDa GST of Clonorchis sinensis in P3221 symmetry

3iso, resolution 1.90Å

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