3jtq

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{{STRUCTURE_3jtq| PDB=3jtq | SCENE= }}
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==Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis==
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===Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis===
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<StructureSection load='3jtq' size='340' side='right' caption='[[3jtq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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{{ABSTRACT_PUBMED_19800869}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3jtq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JTQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2adv|2adv]], [[2ae4|2ae4]], [[2ae3|2ae3]], [[3jtr|3jtr]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaryl-7-aminocephalosporanic-acid_acylase Glutaryl-7-aminocephalosporanic-acid acylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.93 3.5.1.93] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jtq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jtq RCSB], [http://www.ebi.ac.uk/pdbsum/3jtq PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/3jtq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cephalosporin acylase (CA), a member of the N-terminal nucleophile hydrolase family, is activated through two steps of intramolecular autoproteolysis, the first mediated by a serine residue, and the second by a glutamate, which releases the pro-segment and produces an active enzyme. In this study, we have determined the crystal structures of mutants which could affect primary or secondary auto-cleavage and of sequential intermediates of a slow-processing mutant at 2.0-2.5A resolutions. The pro-segments of the mutants undergo dynamic conformational changes during activation and adopt surprisingly different loop conformations from one another. However, the autoproteolytic site was found to form a catalytically competent conformation with a solvent water molecule, which was essentially conserved in the CA mutants.
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==About this Structure==
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Structural features of cephalosporin acylase reveal the basis of autocatalytic activation.,Cho KJ, Kim JK, Lee JH, Shin HJ, Park SS, Kim KH Biochem Biophys Res Commun. 2009 Dec 11;390(2):342-8. Epub 2009 Oct 2. PMID:19800869<ref>PMID:19800869</ref>
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[[3jtq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTQ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:019800869</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Glutaryl-7-aminocephalosporanic-acid acylase]]
[[Category: Glutaryl-7-aminocephalosporanic-acid acylase]]
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[[Category: Pseudomonas sp.]]
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[[Category: Pseudomonas sp]]
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[[Category: Cho, K J.]]
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[[Category: Cho, K J]]
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[[Category: Kim, J K.]]
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[[Category: Kim, J K]]
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[[Category: Kim, K H.]]
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[[Category: Kim, K H]]
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[[Category: Lee, J H.]]
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[[Category: Lee, J H]]
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[[Category: Park, S S.]]
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[[Category: Park, S S]]
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[[Category: Shin, H J.]]
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[[Category: Shin, H J]]
[[Category: Autoproteolysis]]
[[Category: Autoproteolysis]]
[[Category: Cephalosporin acylase]]
[[Category: Cephalosporin acylase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 16:31, 18 December 2014

Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis

3jtq, resolution 2.20Å

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