3izg
From Proteopedia
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| - | + | ==Bacteriophage T7 prohead shell EM-derived atomic model== | |
| - | + | <StructureSection load='3izg' size='340' side='right' caption='[[3izg]], [[Resolution|resolution]] 10.90Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3izg]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IZG FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3izg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3izg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3izg RCSB], [http://www.ebi.ac.uk/pdbsum/3izg PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Maturation of dsDNA bacteriophages involves assembling the virus prohead from a limited set of structural components, followed by rearrangements required for the stability that is necessary for infecting a host under challenging environmental conditions. Here, we determine the mature capsid structure of T7 at 1 nm resolution by cryo electron microscopy, and compare it with the prohead to reveal the molecular basis of T7 shell maturation. The mature capsid presents an expanded and thinner shell, with a drastic rearrangement of the major protein monomers that increases in their interacting surfaces, in turn resulting in a new bonding lattice. The rearrangements include tilting, in-plane rotation and radial expansion of the subunits, as well as a relative bending of the A and P-domains of each subunit. The unique features of this shell transformation, which does not employ the accessory proteins, inserted domains, or molecular interactions observed in other phages, suggest a simple capsid assembling strategy that may have appeared early in the evolution of these viruses. | ||
| - | + | Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7.,Ionel A, Velazquez-Muriel JA, Luque D, Cuervo A, Caston JR, Valpuesta JM, Martin-Benito J, Carrascosa JL J Biol Chem. 2010 Oct 20. PMID:20962334<ref>PMID:20962334</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Enterobacteria phage t7]] | [[Category: Enterobacteria phage t7]] | ||
| - | [[Category: Agirrezabala, X | + | [[Category: Agirrezabala, X]] |
| - | [[Category: Carrascosa, J L | + | [[Category: Carrascosa, J L]] |
| - | [[Category: Caston, J R | + | [[Category: Caston, J R]] |
| - | [[Category: Cuervo, A | + | [[Category: Cuervo, A]] |
| - | [[Category: Ionel, A | + | [[Category: Ionel, A]] |
| - | [[Category: Luque, D | + | [[Category: Luque, D]] |
| - | [[Category: Martin-Benito, J | + | [[Category: Martin-Benito, J]] |
| - | [[Category: Valpuesta, J M | + | [[Category: Valpuesta, J M]] |
| - | [[Category: Velazquez-Muriel, J A | + | [[Category: Velazquez-Muriel, J A]] |
[[Category: Bacteriophage]] | [[Category: Bacteriophage]] | ||
[[Category: Capsid maturation]] | [[Category: Capsid maturation]] | ||
Revision as of 17:41, 18 December 2014
Bacteriophage T7 prohead shell EM-derived atomic model
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