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| - | [[Image:2dtv.jpg|left|200px]]<br /><applet load="2dtv" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[2zg5]] This PDB entry is obsolete and replaced by 2zg5 |
| - | caption="2dtv, resolution 2.26Å" />
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| - | '''Crystal structure of LysN, alpha-aminoadipate aminotransferase, from Thermus thermophilus HB27'''<br />
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| - | ==Overview==
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| - | The extremely thermophilic bacterium Thermus thermophilus HB27 synthesizes lysine through alpha-aminoadipate (AAA). In this study, a T. thermophilus gene encoding the enzyme that catalyses transamination of AAA was cloned as a mammalian kynurenine/AAA aminotransferase (Kat2) gene homologue. A T. thermophilus mutant with disruption of the Kat2 homologue required a longer lag phase for growth and showed slower growth in minimal medium. Furthermore, addition of AAA or lysine shortened the lag phase and improved the growth rate. The Kat2 homologue was therefore termed lysN. LysN recognizes not only 2-oxoadipate, an intermediate of lysine biosynthesis, but also 2-oxoisocaproate, 2-oxoisovalerate and 2-oxo-3-methylvalerate, intermediates of leucine, valine and isoleucine biosyntheses, respectively, along with oxaloacetate, a compound in the TCA cycle, as an amino acceptor. These results suggest multiple roles of LysN in several cellular metabolic pathways including lysine and branched-chain amino acid biosyntheses.
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| - | ==About this Structure==
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| - | 2DTV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=PLP:'>PLP</scene> and <scene name='pdbligand=LEU:'>LEU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/2-aminoadipate_transaminase 2-aminoadipate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.39 2.6.1.39] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DTV OCA].
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| - | ==Reference==
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| - | alpha-Aminoadipate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus., Miyazaki T, Miyazaki J, Yamane H, Nishiyama M, Microbiology. 2004 Jul;150(Pt 7):2327-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15256574 15256574]
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| - | [[Category: 2-aminoadipate transaminase]]
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| - | [[Category: Single protein]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Fushinobu, S.]]
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| - | [[Category: Kuzuyama, T.]]
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| - | [[Category: Miyagawa, T.]]
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| - | [[Category: Miyazaki, T.]]
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| - | [[Category: Nishiyama, M.]]
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| - | [[Category: Tomita, T.]]
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| - | [[Category: LEU]]
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| - | [[Category: PLP]]
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| - | [[Category: alpha-aminoadipate aminotransferase]]
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| - | [[Category: substrate specificity]]
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| - | [[Category: thermus thermophilus]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:02:33 2008''
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