2dtw
From Proteopedia
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| - | [[Image:2dtw.gif|left|200px]] | + | [[Image:2dtw.gif|left|200px]] |
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| - | '''Crystal Structure of basic winged bean lectin in complex with 2Me-O-D-Galactose''' | + | {{Structure |
| + | |PDB= 2dtw |SIZE=350|CAPTION= <scene name='initialview01'>2dtw</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=2GS:2-O-METHYL-ALPHA-D-GALACTOPYRANOSE'>2GS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of basic winged bean lectin in complex with 2Me-O-D-Galactose''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DTW is a [ | + | 2DTW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DTW OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1319-24. Epub 2006, Oct 18. PMID:[http:// | + | Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1319-24. Epub 2006, Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17057334 17057334] |
[[Category: Psophocarpus tetragonolobus]] | [[Category: Psophocarpus tetragonolobus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: legume lectin]] | [[Category: legume lectin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:31:27 2008'' |
Revision as of 14:31, 20 March 2008
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| , resolution 2.40Å | |||||||
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| Ligands: | , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of basic winged bean lectin in complex with 2Me-O-D-Galactose
Overview
The crystal structure of the complexes of basic winged-bean lectin with galactose, 2-methoxygalactose, N-acetylgalactosamine and methyl-alpha-N-acetylgalactosamine have been determined. Lectin-sugar interactions involve four hydrogen bonds and a stacking interaction in all of the complexes. In addition, an N-H...O hydrogen bond involving the hydroxyl group at C2 exists in the galactose and 2-methoxygalactose complexes. An additional hydrophobic interaction involving the methyl group in the latter leads to the higher affinity of the methyl derivative. In the lectin-N-acetylgalactosamine complex the N-H...O hydrogen bond is lost, but a compensatory hydrogen bond is formed involving the O atom of the acetamido group. In addition, the CH(3) moiety of the acetamido group is involved in hydrophobic interactions. Consequently, the 2-methyl and acetamido derivatives of galactose have nearly the same affinity for the lectin. The methyl group alpha-linked to the galactose takes part in additional hydrophobic interactions. Therefore, methyl-alpha-N-acetylgalactosamine has a higher affinity than N-acetylgalactosamine for the lectin. The structures of basic winged-bean lectin-sugar complexes provide a framework for examining the relative affinity of galactose and galactosamine for the lectins that bind to them. The complexes also lead to a structural explanation for the blood-group specificity of basic winged-bean lectin.
About this Structure
2DTW is a Single protein structure of sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA.
Reference
Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1319-24. Epub 2006, Oct 18. PMID:17057334
Page seeded by OCA on Thu Mar 20 16:31:27 2008
Categories: Psophocarpus tetragonolobus | Single protein | Katiyar, S. | Kulkarni, K A. | Suguna, K. | Surolia, A. | Vijayan, M. | 2GS | CA | MN | NAG | Agglutinin | Glycosylated protein | Legume lectin
