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3k64

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{{STRUCTURE_3k64| PDB=3k64 | SCENE= }}
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==Crystal structure of FBF-2/fem-3 PME complex==
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===Crystal structure of FBF-2/fem-3 PME complex===
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<StructureSection load='3k64' size='340' side='right' caption='[[3k64]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_19901328}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3k64]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K64 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K64 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3k5q|3k5q]], [[3k5y|3k5y]], [[3k5z|3k5z]], [[3k61|3k61]], [[3k62|3k62]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">F21H12.5, fbf-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k64 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k64 RCSB], [http://www.ebi.ac.uk/pdbsum/3k64 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k6/3k64_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Caenorhabditis elegans fem-3 binding factor (FBF) is a founding member of the PUMILIO/FBF (PUF) family of mRNA regulatory proteins. It regulates multiple mRNAs critical for stem cell maintenance and germline development. Here, we report crystal structures of FBF in complex with 6 different 9-nt RNA sequences, including elements from 4 natural mRNAs. These structures reveal that FBF binds to conserved bases at positions 1-3 and 7-8. The key specificity determinant of FBF vs. other PUF proteins lies in positions 4-6. In FBF/RNA complexes, these bases stack directly with one another and turn away from the RNA-binding surface. A short region of FBF is sufficient to impart its unique specificity and lies directly opposite the flipped bases. We suggest that this region imposes a flattened curvature on the protein; hence, the requirement for the additional nucleotide. The principles of FBF/RNA recognition suggest a general mechanism by which PUF proteins recognize distinct families of RNAs yet exploit very nearly identical atomic contacts in doing so.
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==Function==
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Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein.,Wang Y, Opperman L, Wickens M, Hall TM Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20186-91. Epub 2009 Nov 9. PMID:19901328<ref>PMID:19901328</ref>
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[[http://www.uniprot.org/uniprot/FBF2_CAEEL FBF2_CAEEL]] Involved in the control of stem cells and sex determination in the C.elegans hermaphrodite germline. May also play a role in the hermaphrodite germline proliferation and oogenesis. Binds specifically to the regulatory region of fem-3 3'-UTR and mediates the sperm/oocyte switch. Negatively regulates gld-3 expression possibly by directly binding to two sites within the gld-3 isoform B 3'-UTR.<ref>PMID:9393998</ref> <ref>PMID:15454534</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3k64]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K64 OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019901328</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Caenorhabditis elegans]]
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[[Category: Hall, T M.T.]]
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[[Category: Hall, T M.T]]
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[[Category: Opperman, L.]]
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[[Category: Opperman, L]]
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[[Category: Wang, Y.]]
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[[Category: Wang, Y]]
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[[Category: Wickens, M.]]
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[[Category: Wickens, M]]
[[Category: Base flipping]]
[[Category: Base flipping]]
[[Category: Base stacking]]
[[Category: Base stacking]]

Revision as of 17:42, 18 December 2014

Crystal structure of FBF-2/fem-3 PME complex

3k64, resolution 2.00Å

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