2dyd
From Proteopedia
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| - | [[Image:2dyd.jpg|left|200px]] | + | [[Image:2dyd.jpg|left|200px]] |
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| - | '''Solution structure of the PABC domain from Triticum aevestium poly(A)-binding protein''' | + | {{Structure |
| + | |PDB= 2dyd |SIZE=350|CAPTION= <scene name='initialview01'>2dyd</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= PABP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4565 Triticum aestivum]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of the PABC domain from Triticum aevestium poly(A)-binding protein''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DYD is a [ | + | 2DYD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DYD OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of the PABC domain from wheat poly (A)-binding protein: an insight into RNA metabolic and translational control in plants., Siddiqui N, Osborne MJ, Gallie DR, Gehring K, Biochemistry. 2007 Apr 10;46(14):4221-31. Epub 2007 Mar 15. PMID:[http:// | + | Solution structure of the PABC domain from wheat poly (A)-binding protein: an insight into RNA metabolic and translational control in plants., Siddiqui N, Osborne MJ, Gallie DR, Gehring K, Biochemistry. 2007 Apr 10;46(14):4221-31. Epub 2007 Mar 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17358048 17358048] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Triticum aestivum]] | [[Category: Triticum aestivum]] | ||
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[[Category: alpha helical protein]] | [[Category: alpha helical protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:33:06 2008'' |
Revision as of 14:33, 20 March 2008
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| Gene: | PABP (Triticum aestivum) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the PABC domain from Triticum aevestium poly(A)-binding protein
Overview
In animals, the PABC domain from poly (A)-binding protein recruits proteins containing a specific interacting motif (PAM-2) to the mRNP complex. These proteins include Paip1, Paip2, and eukaryotic release factor 3 (eRF3), all of which regulate PABP function in translation. The following reports the solution structure of PABC from Triticum avestium (wheat) poly (A)-binding protein determined by NMR spectroscopy. Wheat PABC (wPABC) is an alpha-helical protein domain, which displays a fold highly similar to the human PABC domain and contains a PAM-2 peptide binding site. Through a bioinformatics search, several plant proteins containing a PAM-2 site were identified including the early response to dehydration protein (ERD-15), which was previously shown to regulate PABP-dependent translation. The plant PAM-2 proteins contain a variety of conserved sequences including a PABP-interacting 1 motif (PAM-1), RNA binding domains, an SMR endonuclease domain, and a poly (A)-nuclease regulatory domain, all of which suggest a function in either translation or mRNA metabolism. The proteins identified are well conserved throughout plant species but have no sequence homologues in metazoans. We show that wPABC binds to the plant PAM-2 motif with high affinity through a conserved mechanism. Overall, our results suggest that plant species have evolved a distinct regulatory mechanism involving novel PABP binding partners.
About this Structure
2DYD is a Single protein structure of sequence from Triticum aestivum. Full crystallographic information is available from OCA.
Reference
Solution structure of the PABC domain from wheat poly (A)-binding protein: an insight into RNA metabolic and translational control in plants., Siddiqui N, Osborne MJ, Gallie DR, Gehring K, Biochemistry. 2007 Apr 10;46(14):4221-31. Epub 2007 Mar 15. PMID:17358048
Page seeded by OCA on Thu Mar 20 16:33:06 2008
