2dzn
From Proteopedia
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| - | [[Image:2dzn.jpg|left|200px]] | + | [[Image:2dzn.jpg|left|200px]] |
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| - | '''Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3''' | + | {{Structure |
| + | |PDB= 2dzn |SIZE=350|CAPTION= <scene name='initialview01'>2dzn</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DZN is a [ | + | 2DZN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DZN OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome., Nakamura Y, Umehara T, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S, Biochem Biophys Res Commun. 2007 Aug 3;359(3):503-9. Epub 2007 May 29. PMID:[http:// | + | Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome., Nakamura Y, Umehara T, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S, Biochem Biophys Res Commun. 2007 Aug 3;359(3):503-9. Epub 2007 May 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17555716 17555716] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
[[Category: a-helical domain]] | [[Category: a-helical domain]] | ||
| - | [[Category: ankyrin | + | [[Category: ankyrin repeat]] |
| - | [[Category: national project on protein structural and functional | + | [[Category: national project on protein structural and functional analyse]] |
[[Category: nppsfa]] | [[Category: nppsfa]] | ||
[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:33:26 2008'' |
Revision as of 14:33, 20 March 2008
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| , resolution 2.20Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3
Overview
The 26S proteasome-dependent protein degradation is an evolutionarily conserved process. The mammalian oncoprotein gankyrin, which associates with S6 of the proteasome, facilitates the degradation of pRb, and thus possibly acts as a bridging factor between the proteasome and its substrates. However, the mechanism of the proteasome-dependent protein degradation in yeast is poorly understood. Here, we report the tertiary structure of the complex between Nas6 and a C-terminal domain of Rpt3, which are the yeast orthologues of gankyrin and S6, respectively. The concave region of Nas6 bound to the alpha-helical domain of Rpt3. The stable interaction between Nas6 and Rpt3 was mediated by intermolecular interactions composed of complementary charged patches. The recognition of Rpt3 by Nas6 in the crystal suggests that Nas6 is indeed a subunit of the 26S proteasome. These results provide a structural basis for the association between Nas6 and the heterohexameric ATPase ring of the proteasome through Rpt3.
About this Structure
2DZN is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome., Nakamura Y, Umehara T, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S, Biochem Biophys Res Commun. 2007 Aug 3;359(3):503-9. Epub 2007 May 29. PMID:17555716
Page seeded by OCA on Thu Mar 20 16:33:26 2008
Categories: Protein complex | Saccharomyces cerevisiae | Padmanabhan, B. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Yokoyama, S. | A-helical domain | Ankyrin repeat | National project on protein structural and functional analyse | Nppsfa | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic
