2e1x

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[[Image:2e1x.jpg|left|200px]]<br /><applet load="2e1x" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2e1x.jpg|left|200px]]
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caption="2e1x" />
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'''NMR structure of the HIV-2 nucleocapsid protein'''<br />
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{{Structure
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|PDB= 2e1x |SIZE=350|CAPTION= <scene name='initialview01'>2e1x</scene>
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''NMR structure of the HIV-2 nucleocapsid protein'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2E1X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E1X OCA].
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2E1X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E1X OCA].
==Reference==
==Reference==
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Structural role of the secondary active domain of HIV-2 NCp8 in multi-functionality., Matsui T, Kodera Y, Miyauchi E, Tanaka H, Endoh H, Komatsu H, Tanaka T, Kohno T, Maeda T, Biochem Biophys Res Commun. 2007 Jul 6;358(3):673-8. Epub 2007 May 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17511966 17511966]
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Structural role of the secondary active domain of HIV-2 NCp8 in multi-functionality., Matsui T, Kodera Y, Miyauchi E, Tanaka H, Endoh H, Komatsu H, Tanaka T, Kohno T, Maeda T, Biochem Biophys Res Commun. 2007 Jul 6;358(3):673-8. Epub 2007 May 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17511966 17511966]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Endoh, H.]]
[[Category: Endoh, H.]]
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[[Category: zinc finger]]
[[Category: zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:05:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:34:11 2008''

Revision as of 14:34, 20 March 2008

Image:2e1x.jpg


PDB ID 2e1x

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Ligands:
Coordinates: save as pdb, mmCIF, xml



NMR structure of the HIV-2 nucleocapsid protein


Overview

Nucleocapsid protein of HIV, containing two CCHC-type zinc fingers connected by a linker, is a multi-functional protein involved in many critical steps of the HIV life cycle. Several in vitro investigations demonstrated that the reactivities of the first zinc finger flanked by the linker of HIV-1 NCp7 and HIV-2 NCp8 were essential for binding to viral RNA, however, that of the second zinc finger flanked by the linker of NCp7 was very weak and non-specific, whereas the part of NCp8 called NCp8-f2, interacted strongly and specifically with viral RNA. In this study, the three-dimensional structure of NCp8-f2 was determined for the first time. Furthermore, we established that NCp8-f2 specifically binds to the stem-loop SD in viral RNA, and that the hydrophobic cleft and the basic residues close to the cleft were essential for specific binding to SD. We discuss the functional significance of NCp8-f2 for NCp8 being a multi-functional protein.

About this Structure

2E1X is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Structural role of the secondary active domain of HIV-2 NCp8 in multi-functionality., Matsui T, Kodera Y, Miyauchi E, Tanaka H, Endoh H, Komatsu H, Tanaka T, Kohno T, Maeda T, Biochem Biophys Res Commun. 2007 Jul 6;358(3):673-8. Epub 2007 May 4. PMID:17511966

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