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3l9x

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{{STRUCTURE_3l9x| PDB=3l9x | SCENE= }}
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==KefC C-terminal domain in complex with KefF and ESG==
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===KefC C-terminal domain in complex with KefF and ESG===
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<StructureSection load='3l9x' size='340' side='right' caption='[[3l9x]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21041667}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3l9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L9X FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ESG:L-GAMMA-GLUTAMYL-S-[(3S)-1-ETHYL-2,5-DIOXOPYRROLIDIN-3-YL]-L-CYSTEINYLGLYCINE'>ESG</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=L9X:L-GAMMA-GLUTAMYL-S-[(3R)-1-ETHYL-2,5-DIOXOPYRROLIDIN-3-YL]-L-CYSTEINYLGLYCINE'>L9X</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3l9w|3l9w]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b0047, JW0046, kefC, trkC, b0046, JW0045, kefF, yabF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l9x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l9x RCSB], [http://www.ebi.ac.uk/pdbsum/3l9x PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Gram negative pathogens are protected against toxic electrophilic compounds by glutathione-gated potassium efflux systems (Kef) that modulate cytoplasmic pH. We have elucidated the mechanism of gating through structural and functional analysis of Escherichia coli KefC. The revealed mechanism can explain how subtle chemical differences in glutathione derivatives can produce opposite effects on channel function. Kef channels are regulated by potassium transport and NAD-binding (KTN) domains that sense both reduced glutathione, which inhibits Kef activity, and glutathione adducts that form during electrophile detoxification and activate Kef. We find that reduced glutathione stabilizes an interdomain association between two KTN folds, whereas large adducts sterically disrupt this interaction. F441 is identified as the pivotal residue discriminating between reduced glutathione and its conjugates. We demonstrate a major structural change on the binding of an activating ligand to a KTN-domain protein. Analysis of the regulatory interactions suggests strategies to disrupt pathogen potassium and pH homeostasis.
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==About this Structure==
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Mechanism of ligand-gated potassium efflux in bacterial pathogens.,Roosild TP, Castronovo S, Healy J, Miller S, Pliotas C, Rasmussen T, Bartlett W, Conway SJ, Booth IR Proc Natl Acad Sci U S A. 2010 Nov 1. PMID:21041667<ref>PMID:21041667</ref>
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[[3l9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L9X OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:021041667</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Roosild, T P.]]
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[[Category: Roosild, T P]]
[[Category: Glutathione]]
[[Category: Glutathione]]
[[Category: Potassium channel regulation]]
[[Category: Potassium channel regulation]]
[[Category: Potassium efflux]]
[[Category: Potassium efflux]]
[[Category: Transport protein]]
[[Category: Transport protein]]

Revision as of 15:19, 18 December 2014

KefC C-terminal domain in complex with KefF and ESG

3l9x, resolution 2.10Å

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