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3kxc

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{{STRUCTURE_3kxc| PDB=3kxc | SCENE= }}
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==Mutant transport protein==
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===Mutant transport protein===
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<StructureSection load='3kxc' size='340' side='right' caption='[[3kxc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20372964}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3kxc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KXC FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2cfh|2cfh]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kxc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kxc RCSB], [http://www.ebi.ac.uk/pdbsum/3kxc PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kx/3kxc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bet3, a transport protein particle component involved in vesicular trafficking, contains a hydrophobic tunnel occupied by a fatty acid linked to cysteine 68. We reported that Bet3 has a unique self-palmitoylating activity. Here we show that mutation of arginine 67 reduced self-palmitoylation of Bet3, but the effect was compensated by increasing the pH. Thus, arginine helps to deprotonate cysteine such that it could function as a nucleophile in the acylation reaction which is supported by the structural analysis of non-acylated Bet3. Using fluorescence spectroscopy we show that long-chain acyl-CoAs bind with micromolar affinity to Bet3, whereas shorter-chain acyl-CoAs do not interact. Mutants with a deleted acylation site or a blocked tunnel bind to Pal-CoA, only the latter with slightly reduced affinity. Bet3 contains three binding sites for Pal-CoA, but their number was reduced to two in the mutant with an obstructed tunnel, indicating that Bet3 contains binding sites on its surface.
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==Function==
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Characterization of the self-palmitoylation activity of the transport protein particle component Bet3.,Kummel D, Walter J, Heck M, Heinemann U, Veit M Cell Mol Life Sci. 2010 Apr 6. PMID:20372964<ref>PMID:20372964</ref>
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[[http://www.uniprot.org/uniprot/TPPC3_HUMAN TPPC3_HUMAN]] May play a role in vesicular transport from endoplasmic reticulum to Golgi. [[http://www.uniprot.org/uniprot/TPC6B_HUMAN TPC6B_HUMAN]] May play a role in vesicular transport from endoplasmic reticulum to Golgi (By similarity).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3kxc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXC OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020372964</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Heinemann, U.]]
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[[Category: Heinemann, U]]
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[[Category: Kummel, D.]]
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[[Category: Kummel, D]]
[[Category: Endoplasmic reticulum]]
[[Category: Endoplasmic reticulum]]
[[Category: Er-golgi transport]]
[[Category: Er-golgi transport]]

Revision as of 15:19, 18 December 2014

Mutant transport protein

3kxc, resolution 2.00Å

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