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2e4i
From Proteopedia
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| - | [[Image:2e4i.gif|left|200px]] | + | [[Image:2e4i.gif|left|200px]] |
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| - | '''Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines''' | + | {{Structure |
| + | |PDB= 2e4i |SIZE=350|CAPTION= <scene name='initialview01'>2e4i</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2E4I is a [ | + | 2E4I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E4I OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of a human telomeric DNA sequence stabilized by 8-bromoguanosine substitutions, as determined by NMR in a K+ solution., Matsugami A, Xu Y, Noguchi Y, Sugiyama H, Katahira M, FEBS J. 2007 Jul;274(14):3545-56. Epub 2007 Jun 11. PMID:[http:// | + | Structure of a human telomeric DNA sequence stabilized by 8-bromoguanosine substitutions, as determined by NMR in a K+ solution., Matsugami A, Xu Y, Noguchi Y, Sugiyama H, Katahira M, FEBS J. 2007 Jul;274(14):3545-56. Epub 2007 Jun 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17561958 17561958] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Katahira, M.]] | [[Category: Katahira, M.]] | ||
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[[Category: telomere]] | [[Category: telomere]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:35:13 2008'' |
Revision as of 14:35, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines
Overview
The structure of human telomeric DNA is controversial; it depends upon the sequence contexts and the methodologies used to determine it. The solution structure in the presence of K(+) is particularly interesting, but the structure is yet to be elucidated, due to possible conformational heterogeneity. Here, a unique strategy is applied to stabilize one such structure in a K(+) solution by substituting guanosines with 8-bromoguanosines at proper positions. The resulting spectra are cleaner and led to determination of the structure at a high atomic resolution. This demonstrates that the application of 8-bromoguanosine is a powerful tool to overcome the difficulty of nucleic acid structure determination arising from conformational heterogeneity. The obtained structure is a mixed-parallel/antiparallel quadruplex. The structure of telomeric DNA was recently reported in another study, in which stabilization was brought about by mutation and resultant additional interactions [Luu KN, Phan AT, Kuryavyi V, Lacroix L & Patel DJ (2006) Structure of the human telomere in K(+) solution: an intramolecular (3+1) G-quadruplex scaffold. J Am Chem Soc 128, 9963-9970]. The structure of the guanine tracts was similar between the two. However, a difference was seen for loops connecting guanine tracts, which may play a role in the higher order arrangement of telomeres. Our structure can be utilized to design a small molecule which stabilizes the quadruplex. This type of molecule is supposed to inhibit a telomerase and thus is expected to be a candidate anticancer drug.
About this Structure
2E4I is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Structure of a human telomeric DNA sequence stabilized by 8-bromoguanosine substitutions, as determined by NMR in a K+ solution., Matsugami A, Xu Y, Noguchi Y, Sugiyama H, Katahira M, FEBS J. 2007 Jul;274(14):3545-56. Epub 2007 Jun 11. PMID:17561958
Page seeded by OCA on Thu Mar 20 16:35:13 2008
Categories: Single protein | Katahira, M. | Matsugami, A. | Noguchi, Y. | Sugiyama, H. | Xu, Y. | Dna | Mixed-parallel/antiparallel | Nmr | Quadruplex | Structure | Telomere
