2m1n
From Proteopedia
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| - | + | ==Solution structure of a chaperone in type III secretion system== | |
| - | + | <StructureSection load='2m1n' size='340' side='right' caption='[[2m1n]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2m1n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_3003 Escherichia coli 3003]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M1N FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EC3003_4066 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=869692 Escherichia coli 3003])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m1n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m1n RCSB], [http://www.ebi.ac.uk/pdbsum/2m1n PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The targeting of type III secretion (TTS) proteins at the injectisome is an important process in bacterial virulence. Nevertheless, how the injectisome specifically recognizes TTS substrates among all bacterial proteins is unknown. A TTS peripheral membrane ATPase protein located at the base of the injectisome has been implicated in the targeting process. We have investigated the targeting of the EspA filament protein and its cognate chaperone, CesAB, to the EscN ATPase of the enteropathogenic E. coli (EPEC). We show that EscN selectively engages the EspA-loaded CesAB but not the unliganded CesAB. Structure analysis revealed that the targeting signal is encoded in a disorder-order structural transition in CesAB that is elicited only upon the binding of its physiological substrate, EspA. Abrogation of the interaction between the CesAB-EspA complex and EscN resulted in severe secretion and infection defects. Additionally, we show that the targeting and secretion signals are distinct and that the two processes are likely regulated by different mechanisms. | ||
| - | + | Substrate-Activated Conformational Switch on Chaperones Encodes a Targeting Signal in Type III Secretion.,Chen L, Ai X, Portaliou AG, Minetti CA, Remeta DP, Economou A, Kalodimos CG Cell Rep. 2013 Mar 28;3(3):709-15. doi: 10.1016/j.celrep.2013.02.025. Epub 2013, Mar 21. PMID:23523349<ref>PMID:23523349</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Escherichia coli 3003]] | [[Category: Escherichia coli 3003]] | ||
| - | [[Category: Chen, L | + | [[Category: Chen, L]] |
| - | [[Category: Economou, A | + | [[Category: Economou, A]] |
| - | [[Category: Kalodimos, C | + | [[Category: Kalodimos, C]] |
[[Category: Chaperone]] | [[Category: Chaperone]] | ||
[[Category: Helix bundle]] | [[Category: Helix bundle]] | ||
Revision as of 12:20, 18 December 2014
Solution structure of a chaperone in type III secretion system
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