4gdf
From Proteopedia
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| - | + | ==A Crystal Structure of SV40 Large T Antigen== | |
| - | + | <StructureSection load='4gdf' size='340' side='right' caption='[[4gdf]], [[Resolution|resolution]] 2.80Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4gdf]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GDF FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gdf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gdf RCSB], [http://www.ebi.ac.uk/pdbsum/4gdf PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The DNA tumor virus Simian virus 40 (SV40) is a model system for studying eukaryotic replication. SV40 large tumor antigen (LTag) is the initiator/helicase that is essential for genome replication. LTag recognizes and assembles at the viral replication origin. We determined the structure of two multidomain LTag subunits bound to origin DNA. The structure reveals that the origin binding domains (OBDs) and Zn and AAA+ domains are involved in origin recognition and assembly. Notably, the OBDs recognize the origin in an unexpected manner. The histidine residues of the AAA+ domains insert into a narrow minor groove region with enhanced negative electrostatic potential. Computational analysis indicates that this region is intrinsically narrow, demonstrating the role of DNA shape readout in origin recognition. Our results provide important insights into the assembly of the LTag initiator/helicase at the replication origin and suggest that histidine contacts with the minor groove serve as a mechanism of DNA shape readout. | ||
| - | + | Mechanism of Origin DNA Recognition and Assembly of an Initiator-Helicase Complex by SV40 Large Tumor Antigen.,Chang YP, Xu M, Machado AC, Yu XJ, Rohs R, Chen XS Cell Rep. 2013 Mar 27. pii: S2211-1247(13)00109-5. doi:, 10.1016/j.celrep.2013.03.002. PMID:23545501<ref>PMID:23545501</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Large T Antigen|Large T Antigen]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
| - | [[Category: Chang, Y P | + | [[Category: Chang, Y P]] |
| - | [[Category: Chen, X S | + | [[Category: Chen, X S]] |
| - | [[Category: Xu, M | + | [[Category: Xu, M]] |
[[Category: Dna replication]] | [[Category: Dna replication]] | ||
[[Category: Helicase]] | [[Category: Helicase]] | ||
Revision as of 11:49, 21 December 2014
A Crystal Structure of SV40 Large T Antigen
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