3ab3

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{{STRUCTURE_3ab3| PDB=3ab3 | SCENE= }}
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==Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13==
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===Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13===
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<StructureSection load='3ab3' size='340' side='right' caption='[[3ab3]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21507947}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ab3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AB3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AB3 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zcb|1zcb]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Gna13, Gna-13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), ARHGEF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ab3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ab3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ab3 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RH-RhoGEFs are a family of guanine nucleotide exchange factors that contain a regulator of G protein signaling homology (RH) domain. The heterotrimeric G protein Galpha(13) stimulates the guanine nucleotide exchange factor (GEF) activity of RH-RhoGEFs, leading to activation of RhoA. The mechanism by which Galpha(13) stimulates the GEF activity of RH-RhoGEFs, such as p115RhoGEF, has not yet been fully elucidated. Here, specific residues in Galpha(13) that mediate activation of p115RhoGEF are identified. Mutation of these residues significantly impairs binding of Galpha(13) to p115RhoGEF as well as stimulation of GEF activity. These data suggest that the exchange activity of p115RhoGEF is stimulated allosterically by Galpha(13) and not through its interaction with a secondary binding site. A crystal structure of Galpha(13) bound to the RH domain of p115RhoGEF is also presented, which differs from a previously crystallized complex with a Galpha(13)-Galpha(i1) chimera. Taken together, these data provide new insight into the mechanism by which p115RhoGEF is activated by Galpha(13).
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==Function==
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Identification of Critical Residues in G{alpha}13 for Stimulation of p115RhoGEF Activity and the Structure of the G{alpha}13-p115RhoGEF Regulator of G Protein Signaling Homology (RH) Domain Complex.,Hajicek N, Kukimoto-Niino M, Mishima-Tsumagari C, Chow CR, Shirouzu M, Terada T, Patel M, Yokoyama S, Kozasa T J Biol Chem. 2011 Jun 10;286(23):20625-36. Epub 2011 Apr 20. PMID:21507947<ref>PMID:21507947</ref>
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[[http://www.uniprot.org/uniprot/GNAI3_MOUSE GNAI3_MOUSE]] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. [[http://www.uniprot.org/uniprot/ARHG1_HUMAN ARHG1_HUMAN]] Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.<ref>PMID:8810315</ref> <ref>PMID:9641915</ref> <ref>PMID:9641916</ref> <ref>PMID:20098430</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3ab3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AB3 OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021507947</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Kozasa, T.]]
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[[Category: Kozasa, T]]
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[[Category: Kukimoto-Niino, M.]]
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[[Category: Kukimoto-Niino, M]]
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[[Category: Mishima, C.]]
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[[Category: Mishima, C]]
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[[Category: Shirouzu, M.]]
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[[Category: Shirouzu, M]]
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[[Category: Yokoyama, S.]]
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[[Category: Yokoyama, S]]
[[Category: Gtp-binding]]
[[Category: Gtp-binding]]
[[Category: Gtpase activation]]
[[Category: Gtpase activation]]

Revision as of 14:21, 18 December 2014

Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13

3ab3, resolution 2.40Å

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