3m6i

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{{STRUCTURE_3m6i| PDB=3m6i | SCENE= }}
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==L-arabinitol 4-dehydrogenase==
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===L-arabinitol 4-dehydrogenase===
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<StructureSection load='3m6i' size='340' side='right' caption='[[3m6i]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20655316}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3m6i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M6I FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NCU00643, NCU00643.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Neurospora crassa])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-arabinitol_4-dehydrogenase L-arabinitol 4-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.12 1.1.1.12] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m6i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m6i RCSB], [http://www.ebi.ac.uk/pdbsum/3m6i PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m6/3m6i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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L-arabinitol 4-dehydrogenase (LAD) catalyzes the conversion of l-arabinitol into l-xylulose with concomitant NAD(+) reduction. It is an essential enzyme in the development of recombinant organisms that convert l-arabinose into fuels and chemicals using the fungal l-arabinose catabolic pathway. Here we report the crystal structure of LAD from the filamentous fungus Neurospora crassa at 2.6 A resolution. In addition, we created a number of site-directed variants of N. crassa LAD that are capable of utilizing NADP(+) as cofactor, yielding the first example of LAD with an almost completely switched cofactor specificity. This work represents the first structural data on any LAD and provides a molecular basis for understanding the existing literature on the substrate specificity and cofactor specificity of this enzyme. The engineered LAD mutants with altered cofactor specificity should be useful for applications in industrial biotechnology.
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==Function==
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Structure and engineering of L-arabinitol 4-dehydrogenase from Neurospora crassa.,Bae B, Sullivan RP, Zhao H, Nair SK J Mol Biol. 2010 Sep 10;402(1):230-40. Epub 2010 Jul 22. PMID:20655316<ref>PMID:20655316</ref>
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[[http://www.uniprot.org/uniprot/Q7SI09_NEUCR Q7SI09_NEUCR]] Catalyzes the NAD-dependent oxidation of L-arabinitol to L-xylulose in the fungal L-arabinose catabolic pathway. L-arabinose catabolism is important for using plant material as a carbon source. Not active on D-arabinitol, D-sorbitol and D-mannitol.<ref>PMID:17938906</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3m6i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M6I OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020655316</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: L-arabinitol 4-dehydrogenase]]
[[Category: L-arabinitol 4-dehydrogenase]]
[[Category: Neurospora crassa]]
[[Category: Neurospora crassa]]
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[[Category: Bae, B.]]
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[[Category: Bae, B]]
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[[Category: Nair, S K.]]
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[[Category: Nair, S K]]
[[Category: Medium chain dehydrogenase/reductase]]
[[Category: Medium chain dehydrogenase/reductase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]

Revision as of 16:32, 18 December 2014

L-arabinitol 4-dehydrogenase

3m6i, resolution 2.60Å

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