4iu8
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of a membrane transporter (selenomethionine derivative)== | |
| - | + | <StructureSection load='4iu8' size='340' side='right' caption='[[4iu8]], [[Resolution|resolution]] 3.11Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4iu8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IU8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4iu9|4iu9]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">narU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iu8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iu8 RCSB], [http://www.ebi.ac.uk/pdbsum/4iu8 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The nitrate/nitrite transporters NarK and NarU play an important role in nitrogen homeostasis in bacteria and belong to the nitrate/nitrite porter family (NNP) of the major facilitator superfamily (MFS) fold. The structure and functional mechanism of NarK and NarU remain unknown. Here, we report the crystal structure of NarU at a resolution of 3.1 A and systematic biochemical characterization. The two molecules of NarU in an asymmetric unit exhibit two distinct conformational states: occluded and partially inward-open. The substrate molecule nitrate appears to be coordinated by four highly conserved, charged, or polar amino acids. Structural and biochemical analyses allowed the identification of key amino acids that are involved in substrate gating and transport. The observed conformational differences of NarU, together with unique sequence features of the NNP family transporters, suggest a transport mechanism that might deviate from the canonical rocker-switch model. | ||
| - | + | Structure and mechanism of a nitrate transporter.,Yan H, Huang W, Yan C, Gong X, Jiang S, Zhao Y, Wang J, Shi Y Cell Rep. 2013 Mar 28;3(3):716-23. doi: 10.1016/j.celrep.2013.03.007. Epub 2013, Mar 21. PMID:23523348<ref>PMID:23523348</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Escherichia coli k-12]] | [[Category: Escherichia coli k-12]] | ||
| - | [[Category: Gong, X | + | [[Category: Gong, X]] |
| - | [[Category: Huang, W | + | [[Category: Huang, W]] |
| - | [[Category: Jiang, S | + | [[Category: Jiang, S]] |
| - | [[Category: Shi, Y | + | [[Category: Shi, Y]] |
| - | [[Category: Wang, J | + | [[Category: Wang, J]] |
| - | [[Category: Yan, C | + | [[Category: Yan, C]] |
| - | [[Category: Yan, H | + | [[Category: Yan, H]] |
| - | [[Category: Zhao, Y | + | [[Category: Zhao, Y]] |
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
[[Category: Mfs fold]] | [[Category: Mfs fold]] | ||
[[Category: Nitrate-nitrite porter family transporter]] | [[Category: Nitrate-nitrite porter family transporter]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||
Revision as of 13:06, 21 December 2014
Crystal structure of a membrane transporter (selenomethionine derivative)
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