4h1p

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_4h1p| PDB=4h1p | SCENE= }}
+
==Use of Europium for SAD Phasing at the Cu K alpha wavelength==
-
===Use of Europium for SAD Phasing at the Cu K alpha wavelength===
+
<StructureSection load='4h1p' size='340' side='right' caption='[[4h1p]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_23295480}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4h1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4eni 4eni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1P FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EU:EUROPIUM+ION'>EU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
 +
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1h87|1h87]]</td></tr>
 +
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h1p RCSB], [http://www.ebi.ac.uk/pdbsum/4h1p PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Europium is shown to be a good anomalous scatterer in SAD phasing for solving the structure of biological macromolecules. The large value of the anomalous contribution of europium, f'' = 11.17 e(-), at the Cu Kalpha wavelength is an advantage in de novo phasing and automated model building. Tetragonal crystals of hen egg-white lysozyme (HEWL) incorporating europium(III) chloride (50 mM) were obtained which diffracted to a resolution of 2.3 A at a wavelength of 1.54 A (Cu Kalpha). The master data set (360 degrees frames) was split and analyzed for anomalous signal-to-noise ratio, multiplicity, completeness, SAD phasing and automated building. The structure solution and model building of the split data sets were carried out using phenix.autosol and phenix.autobuild. The contributions of the Eu ions to SAD phasing using in-house data collection are discussed. This study revealed successful lysozyme phasing by SAD using laboratory-source data involving Eu ions, which are mainly coordinated by the side chains of Asn46, Asp52 and Asp101 together with some water molecules.
-
==Function==
+
Use of europium ions for SAD phasing of lysozyme at the Cu Kalpha wavelength.,Vijayakumar B, Velmurugan D Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jan 1;69(Pt 1):20-4. doi: , 10.1107/S1744309112047562. Epub 2012 Dec 25. PMID:23295480<ref>PMID:23295480</ref>
-
[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[4h1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4eni 4eni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1P OCA].
+
</div>
==See Also==
==See Also==
-
*[[Hen Egg-White (HEW) Lysozyme|Hen Egg-White (HEW) Lysozyme]]
+
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
-
[[Category: Velmurugan, D.]]
+
[[Category: Velmurugan, D]]
-
[[Category: Vijayakumar, B.]]
+
[[Category: Vijayakumar, B]]
[[Category: Catalytic activity]]
[[Category: Catalytic activity]]
[[Category: Hewl]]
[[Category: Hewl]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Lysozyme activity]]
[[Category: Lysozyme activity]]

Revision as of 11:09, 21 December 2014

Use of Europium for SAD Phasing at the Cu K alpha wavelength

4h1p, resolution 2.30Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4h1p"
Personal tools