2f1m
From Proteopedia
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| - | [[Image:2f1m.gif|left|200px]] | + | [[Image:2f1m.gif|left|200px]] |
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| - | '''Conformational flexibility in the multidrug efflux system protein AcrA''' | + | {{Structure |
| + | |PDB= 2f1m |SIZE=350|CAPTION= <scene name='initialview01'>2f1m</scene>, resolution 2.71Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= acrA, lir, mtcA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Conformational flexibility in the multidrug efflux system protein AcrA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2F1M is a [ | + | 2F1M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F1M OCA]. |
==Reference== | ==Reference== | ||
| - | Conformational flexibility in the multidrug efflux system protein AcrA., Mikolosko J, Bobyk K, Zgurskaya HI, Ghosh P, Structure. 2006 Mar;14(3):577-87. PMID:[http:// | + | Conformational flexibility in the multidrug efflux system protein AcrA., Mikolosko J, Bobyk K, Zgurskaya HI, Ghosh P, Structure. 2006 Mar;14(3):577-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16531241 16531241] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lipoyl domain]] | [[Category: lipoyl domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:46:59 2008'' |
Revision as of 14:47, 20 March 2008
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| , resolution 2.71Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | acrA, lir, mtcA (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Conformational flexibility in the multidrug efflux system protein AcrA
Overview
Intrinsic resistance to multiple drugs in many gram-negative bacterial pathogens is conferred by resistance nodulation cell division efflux pumps, which are composed of three essential components as typified by the extensively characterized Escherichia coli AcrA-AcrB-TolC system. The inner membrane drug:proton antiporter AcrB and the outer membrane channel TolC export chemically diverse compounds out of the bacterial cell, and require the activity of the third component, the periplasmic protein AcrA. The crystal structures of AcrB and TolC have previously been determined, and we complete the molecular picture of the efflux system by presenting the structure of a stable fragment of AcrA. The AcrA fragment resembles the elongated sickle shape of its homolog Pseudomonas aeruginosa MexA, being composed of three domains: beta-barrel, lipoyl, and alpha-helical hairpin. Notably, unsuspected conformational flexibility in the alpha-helical hairpin domain of AcrA is observed, which has potential mechanistic significance in coupling between AcrA conformations and TolC channel opening.
About this Structure
2F1M is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Conformational flexibility in the multidrug efflux system protein AcrA., Mikolosko J, Bobyk K, Zgurskaya HI, Ghosh P, Structure. 2006 Mar;14(3):577-87. PMID:16531241
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