2f5o
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2f5o.gif|left|200px]] | + | [[Image:2f5o.gif|left|200px]] |
| - | + | ||
| - | '''MutM crosslinked to undamaged DNA sampling G:C base pair IC3''' | + | {{Structure |
| + | |PDB= 2f5o |SIZE=350|CAPTION= <scene name='initialview01'>2f5o</scene>, resolution 2.05Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-formamidopyrimidine_glycosylase DNA-formamidopyrimidine glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.23 3.2.2.23] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''MutM crosslinked to undamaged DNA sampling G:C base pair IC3''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2F5O is a [ | + | 2F5O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5O OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of a DNA glycosylase searching for lesions., Banerjee A, Santos WL, Verdine GL, Science. 2006 Feb 24;311(5764):1153-7. PMID:[http:// | + | Structure of a DNA glycosylase searching for lesions., Banerjee A, Santos WL, Verdine GL, Science. 2006 Feb 24;311(5764):1153-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16497933 16497933] |
[[Category: DNA-formamidopyrimidine glycosylase]] | [[Category: DNA-formamidopyrimidine glycosylase]] | ||
[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
| Line 24: | Line 33: | ||
[[Category: dna repair]] | [[Category: dna repair]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:48:22 2008'' |
Revision as of 14:48, 20 March 2008
| |||||||
| , resolution 2.05Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | DNA-formamidopyrimidine glycosylase, with EC number 3.2.2.23 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
MutM crosslinked to undamaged DNA sampling G:C base pair IC3
Overview
DNA glycosylases must interrogate millions of base pairs of undamaged DNA in order to locate and then excise one damaged nucleobase. The nature of this search process remains poorly understood. Here we report the use of disulfide cross-linking (DXL) technology to obtain structures of a bacterial DNA glycosylase, MutM, interrogating undamaged DNA. These structures, solved to 2.0 angstrom resolution, reveal the nature of the search process: The protein inserts a probe residue into the helical stack and severely buckles the target base pair, which remains intrahelical. MutM therefore actively interrogates the intact DNA helix while searching for damage.
About this Structure
2F5O is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
Structure of a DNA glycosylase searching for lesions., Banerjee A, Santos WL, Verdine GL, Science. 2006 Feb 24;311(5764):1153-7. PMID:16497933
Page seeded by OCA on Thu Mar 20 16:48:22 2008
