3nnl

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{{STRUCTURE_3nnl| PDB=3nnl | SCENE= }}
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==Halogenase domain from CurA module (crystal form III)==
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===Halogenase domain from CurA module (crystal form III)===
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<StructureSection load='3nnl' size='340' side='right' caption='[[3nnl]], [[Resolution|resolution]] 2.88&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20660778}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3nnl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NNL FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nnf|3nnf]], [[3nnj|3nnj]], [[3nnm|3nnm]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">curA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158786 Lyngbya majuscula])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nnl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nnl RCSB], [http://www.ebi.ac.uk/pdbsum/3nnl PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nn/3nnl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The CurA halogenase (Hal) catalyzes a cryptic chlorination leading to cyclopropane ring formation in the synthesis of the natural product curacin A. Hal belongs to a family of enzymes that use Fe(2+), O(2) and alpha-ketoglutarate (alphaKG) to perform a variety of halogenation reactions in natural product biosynthesis. Crystal structures of the enzyme in five ligand states reveal strikingly different open and closed conformations dependent on alphaKG binding. The open form represents ligand-free enzyme, preventing substrate from entering the active site until both alphaKG and chloride are bound, while the closed form represents the holoenzyme with alphaKG and chloride coordinated to iron. Candidate amino acid residues involved in substrate recognition were identified by site-directed mutagenesis. These new structures provide direct evidence of a conformational switch driven by alphaKG leading to chlorination of an early pathway intermediate.
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==About this Structure==
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Conformational switch triggered by alpha-ketoglutarate in a halogenase of curacin A biosynthesis.,Khare D, Wang B, Gu L, Razelun J, Sherman DH, Gerwick WH, Hakansson K, Smith JL Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14099-104. Epub 2010 Jul 26. PMID:20660778<ref>PMID:20660778</ref>
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[[3nnl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NNL OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020660778</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Lyngbya majuscula]]
[[Category: Lyngbya majuscula]]
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[[Category: Khare, D.]]
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[[Category: Khare, D]]
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[[Category: Smith, J L.]]
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[[Category: Smith, J L]]
[[Category: Biosynthetic protein]]
[[Category: Biosynthetic protein]]
[[Category: Catalyzes a cryptic chlorination]]
[[Category: Catalyzes a cryptic chlorination]]

Revision as of 06:34, 19 December 2014

Halogenase domain from CurA module (crystal form III)

3nnl, resolution 2.88Å

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