2f98
From Proteopedia
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| - | [[Image:2f98.gif|left|200px]] | + | [[Image:2f98.gif|left|200px]] |
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| - | '''Crystal structure of the polyketide cyclase AknH with bound substrate and product analogue: implications for catalytic mechanism and product stereoselectivity.''' | + | {{Structure |
| + | |PDB= 2f98 |SIZE=350|CAPTION= <scene name='initialview01'>2f98</scene>, resolution 2.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=NGV:METHYL 5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the polyketide cyclase AknH with bound substrate and product analogue: implications for catalytic mechanism and product stereoselectivity.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2F98 is a [ | + | 2F98 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_galilaeus Streptomyces galilaeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F98 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the polyketide cyclase AknH with bound substrate and product analogue: implications for catalytic mechanism and product stereoselectivity., Kallio P, Sultana A, Niemi J, Mantsala P, Schneider G, J Mol Biol. 2006 Mar 17;357(1):210-20. Epub 2006 Jan 6. PMID:[http:// | + | Crystal structure of the polyketide cyclase AknH with bound substrate and product analogue: implications for catalytic mechanism and product stereoselectivity., Kallio P, Sultana A, Niemi J, Mantsala P, Schneider G, J Mol Biol. 2006 Mar 17;357(1):210-20. Epub 2006 Jan 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16414075 16414075] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces galilaeus]] | [[Category: Streptomyces galilaeus]] | ||
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[[Category: stereoselectivity]] | [[Category: stereoselectivity]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:49:36 2008'' |
Revision as of 14:49, 20 March 2008
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| , resolution 2.10Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the polyketide cyclase AknH with bound substrate and product analogue: implications for catalytic mechanism and product stereoselectivity.
Overview
AknH is a small polyketide cyclase that catalyses the closure of the fourth carbon ring in aclacinomycin biosynthesis in Streptomyces galilaeus, converting aklanonic acid methyl ester to aklaviketone. The crystal structure analysis of this enzyme, in complex with substrate and product analogue, showed that it is closely related in fold and mechanism to the polyketide cyclase SnoaL that catalyses the corresponding reaction in the biosynthesis of nogalamycin. Similarity is also apparent at a functional level as AknH can convert nogalonic acid methyl ester, the natural substrate of SnoaL, to auraviketone in vitro and in constructs in vivo. Despite the conserved structural and mechanistic features between these enzymes, the reaction products of AknH and SnoaL are stereochemically distinct. Supplied with the same substrate, AknH yields a C9-R product, like most members of this family of polyketide cyclases, whereas the product of SnoaL has the opposite C9-S stereochemistry. A comparison of high-resolution crystal structures of the two enzymes combined with in vitro mutagenesis studies revealed two critical amino acid substitutions in the active sites, which contribute to product stereoselectivity in AknH. Replacement of residues Tyr15 and Asn51 of AknH, located in the vicinity of the main catalytic residue Asp121, by their SnoaL counter-parts phenylalanine and leucine, respectively, results in a complete loss of product stereoselectivity.
About this Structure
2F98 is a Single protein structure of sequence from Streptomyces galilaeus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the polyketide cyclase AknH with bound substrate and product analogue: implications for catalytic mechanism and product stereoselectivity., Kallio P, Sultana A, Niemi J, Mantsala P, Schneider G, J Mol Biol. 2006 Mar 17;357(1):210-20. Epub 2006 Jan 6. PMID:16414075
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