3nmv

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{{STRUCTURE_3nmv| PDB=3nmv | SCENE= }}
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==Crystal structure of pyrabactin-bound abscisic acid receptor PYL2 mutant A93F in complex with type 2C protein phosphatase ABI2==
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===Crystal structure of pyrabactin-bound abscisic acid receptor PYL2 mutant A93F in complex with type 2C protein phosphatase ABI2===
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<StructureSection load='3nmv' size='340' side='right' caption='[[3nmv]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20729862}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3nmv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NMV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NMV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PYV:4-BROMO-N-(PYRIDIN-2-YLMETHYL)NAPHTHALENE-1-SULFONAMIDE'>PYV</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nmh|3nmh]], [[3nmn|3nmn]], [[3nmp|3nmp]], [[3nmt|3nmt]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PYL2, RCAR14, At2g26040, T19L18.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), ABI2, At5g57050, MHM17.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nmv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nmv RCSB], [http://www.ebi.ac.uk/pdbsum/3nmv PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nm/3nmv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The phytohormone abscisic acid (ABA) functions through a family of fourteen PYR/PYL receptors, which were identified by resistance to pyrabactin, a synthetic inhibitor of seed germination. ABA activates these receptors to inhibit type 2C protein phosphatases, such as ABI1, yet it remains unclear whether these receptors can be antagonized. Here we demonstrate that pyrabactin is an agonist of PYR1 and PYL1 but is unexpectedly an antagonist of PYL2. Crystal structures of the PYL2-pyrabactin and PYL1-pyrabactin-ABI1 complexes reveal the mechanism responsible for receptor-selective activation and inhibition, which enables us to design mutations that convert PYL1 to a pyrabactin-inhibited receptor and PYL2 to a pyrabactin-activated receptor and to identify new pyrabactin-based ABA receptor agonists. Together, our results establish a new concept of ABA receptor antagonism, illustrate its underlying mechanisms and provide a rational framework for discovering novel ABA receptor ligands.
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==Function==
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Identification and mechanism of ABA receptor antagonism.,Melcher K, Xu Y, Ng LM, Zhou XE, Soon FF, Chinnusamy V, Suino-Powell KM, Kovach A, Tham FS, Cutler SR, Li J, Yong EL, Zhu JK, Xu HE Nat Struct Mol Biol. 2010 Sep;17(9):1102-8. Epub 2010 Aug 22. PMID:20729862<ref>PMID:20729862</ref>
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[[http://www.uniprot.org/uniprot/PYL2_ARATH PYL2_ARATH]] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA.<ref>PMID:19898420</ref> <ref>PMID:19893533</ref> [[http://www.uniprot.org/uniprot/P2C77_ARATH P2C77_ARATH]] Repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), high light stress, response to glucose, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as H(2)O(2) and oxidative burst in response to ABA and dehydration. Controls negatively fibrillin that is involved in mediating ABA-induced photoprotection. May be implicated in ABA content regulation. Involved in acquired thermotolerance of root growth and seedling survival. Required for the Erwinia amylovora harpin-induced (HrpN) drought tolerance. Involved in the hydrotropic response.<ref>PMID:9165752</ref> <ref>PMID:12232276</ref> <ref>PMID:8787023</ref> <ref>PMID:9108297</ref> <ref>PMID:9090884</ref> <ref>PMID:9276963</ref> <ref>PMID:10488243</ref> <ref>PMID:10950871</ref> <ref>PMID:10872217</ref> <ref>PMID:11701885</ref> <ref>PMID:11208021</ref> <ref>PMID:11707572</ref> <ref>PMID:12194854</ref> <ref>PMID:12447533</ref> <ref>PMID:12119381</ref> <ref>PMID:14596925</ref> <ref>PMID:12609042</ref> <ref>PMID:14576281</ref> <ref>PMID:15599761</ref> <ref>PMID:15923322</ref> <ref>PMID:16339784</ref> <ref>PMID:16571665</ref> <ref>PMID:18278579</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3nmv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NMV OCA].
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</div>
==See Also==
==See Also==
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*[[ABA-regulated Protein Phosphatase 2C|ABA-regulated Protein Phosphatase 2C]]
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*[[PYR/PYL/RCAR family of ABA receptors|PYR/PYL/RCAR family of ABA receptors]]
*[[Protein phosphatase|Protein phosphatase]]
*[[Protein phosphatase|Protein phosphatase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020729862</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Phosphoprotein phosphatase]]
[[Category: Phosphoprotein phosphatase]]
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[[Category: Kovach, A.]]
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[[Category: Kovach, A]]
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[[Category: Li, J.]]
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[[Category: Li, J]]
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[[Category: Melcher, K.]]
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[[Category: Melcher, K]]
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[[Category: Ng, L M.]]
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[[Category: Ng, L M]]
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[[Category: Soon, F F.]]
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[[Category: Soon, F F]]
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[[Category: Suino-Powell, K M.]]
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[[Category: Suino-Powell, K M]]
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[[Category: Xu, H E.]]
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[[Category: Xu, H E]]
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[[Category: Xu, Y.]]
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[[Category: Xu, Y]]
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[[Category: Yong, E L.]]
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[[Category: Yong, E L]]
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[[Category: Zhou, X E.]]
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[[Category: Zhou, X E]]
[[Category: Abscisic acid receptor]]
[[Category: Abscisic acid receptor]]
[[Category: Helix-grip fold]]
[[Category: Helix-grip fold]]

Revision as of 07:23, 19 December 2014

Crystal structure of pyrabactin-bound abscisic acid receptor PYL2 mutant A93F in complex with type 2C protein phosphatase ABI2

3nmv, resolution 2.10Å

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