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2fa1
From Proteopedia
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| - | [[Image:2fa1.gif|left|200px]] | + | [[Image:2fa1.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of PhnF C-terminal domain''' | + | {{Structure |
| + | |PDB= 2fa1 |SIZE=350|CAPTION= <scene name='initialview01'>2fa1</scene>, resolution 1.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= phnF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of PhnF C-terminal domain''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FA1 is a [ | + | 2FA1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FA1 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural characterization of GntR/HutC family signaling domain., Gorelik M, Lunin VV, Skarina T, Savchenko A, Protein Sci. 2006 Jun;15(6):1506-11. Epub 2006 May 2. PMID:[http:// | + | Structural characterization of GntR/HutC family signaling domain., Gorelik M, Lunin VV, Skarina T, Savchenko A, Protein Sci. 2006 Jun;15(6):1506-11. Epub 2006 May 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16672238 16672238] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: effector binding domain]] | [[Category: effector binding domain]] | ||
[[Category: mcsg]] | [[Category: mcsg]] | ||
| - | [[Category: midwest center for structural | + | [[Category: midwest center for structural genomic]] |
[[Category: pnhf]] | [[Category: pnhf]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:49:55 2008'' |
Revision as of 14:50, 20 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | phnF (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of PhnF C-terminal domain
Overview
The crystal structure of Escherichia coli PhnF C-terminal domain (C-PhnF) was solved at 1.7 A resolution by the single wavelength anomalous dispersion (SAD) method. The PhnF protein belongs to the HutC subfamily of the large GntR transcriptional regulator family. Members of this family share similar N-terminal DNA-binding domains, but are divided into four subfamilies according to their heterogenic C-terminal domains, which are involved in effector binding and oligomerization. The C-PhnF structure provides for the first time the scaffold of this domain for the HutC subfamily, which covers about 31% of GntR-like regulators. The structure represents a mixture of alpha-helices and beta-strands, with a six-stranded antiparallel beta-sheet at the core. C-PhnF monomers form a dimer by establishing interdomain eight-strand beta-sheets that include core antiparallel and N-terminal two-strand parallel beta-sheets from each monomer. C-PhnF shares strong structural similarity with the chorismate lyase fold, which features a buried active site locked behind two helix-turn-helix loops. The structural comparison of the C-PhnF and UbiC proteins allows us to propose that a similar site in the PhnF structure is adapted for effector binding.
About this Structure
2FA1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural characterization of GntR/HutC family signaling domain., Gorelik M, Lunin VV, Skarina T, Savchenko A, Protein Sci. 2006 Jun;15(6):1506-11. Epub 2006 May 2. PMID:16672238
Page seeded by OCA on Thu Mar 20 16:49:55 2008
Categories: Escherichia coli | Single protein | Edwards, A M. | Gorelik, M. | Lunin, V V. | MCSG, Midwest Center for Structural Genomics. | Nocek, B P. | Savchenko, A. | Skarina, T. | BDF | Apc5558 | Effector binding domain | Mcsg | Midwest center for structural genomic | Pnhf | Protein structure initiative | Psi | Regulator | Transcription
