2fca
From Proteopedia
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| - | [[Image:2fca.gif|left|200px]] | + | [[Image:2fca.gif|left|200px]] |
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| - | '''The structure of BsTrmB''' | + | {{Structure |
| + | |PDB= 2fca |SIZE=350|CAPTION= <scene name='initialview01'>2fca</scene>, resolution 2.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=K:POTASSIUM ION'>K</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/tRNA_(guanine-N(7)-)-methyltransferase tRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.33 2.1.1.33] | ||
| + | |GENE= trmB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | }} | ||
| + | |||
| + | '''The structure of BsTrmB''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FCA is a [ | + | 2FCA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCA OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase., Zegers I, Gigot D, van Vliet F, Tricot C, Aymerich S, Bujnicki JM, Kosinski J, Droogmans L, Nucleic Acids Res. 2006 Apr 5;34(6):1925-34. Print 2006. PMID:[http:// | + | Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase., Zegers I, Gigot D, van Vliet F, Tricot C, Aymerich S, Bujnicki JM, Kosinski J, Droogmans L, Nucleic Acids Res. 2006 Apr 5;34(6):1925-34. Print 2006. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16600901 16600901] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: methyltransferase]] | [[Category: methyltransferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:50:37 2008'' |
Revision as of 14:50, 20 March 2008
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| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | trmB (Bacillus subtilis) | ||||||
| Activity: | tRNA (guanine-N(7)-)-methyltransferase, with EC number 2.1.1.33 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The structure of BsTrmB
Overview
The structure of Bacillus subtilis TrmB (BsTrmB), the tRNA (m7G46) methyltransferase, was determined at a resolution of 2.1 A. This is the first structure of a member of the TrmB family to be determined by X-ray crystallography. It reveals a unique variant of the Rossmann-fold methyltransferase (RFM) structure, with the N-terminal helix folded on the opposite site of the catalytic domain. The architecture of the active site and a computational docking model of BsTrmB in complex with the methyl group donor S-adenosyl-L-methionine and the tRNA substrate provide an explanation for results from mutagenesis studies of an orthologous enzyme from Escherichia coli (EcTrmB). However, unlike EcTrmB, BsTrmB is shown here to be dimeric both in the crystal and in solution. The dimer interface has a hydrophobic core and buries a potassium ion and five water molecules. The evolutionary analysis of the putative interface residues in the TrmB family suggests that homodimerization may be a specific feature of TrmBs from Bacilli, which may represent an early stage of evolution to an obligatory dimer.
About this Structure
2FCA is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase., Zegers I, Gigot D, van Vliet F, Tricot C, Aymerich S, Bujnicki JM, Kosinski J, Droogmans L, Nucleic Acids Res. 2006 Apr 5;34(6):1925-34. Print 2006. PMID:16600901
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