2fco
From Proteopedia
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| - | [[Image:2fco.gif|left|200px]] | + | [[Image:2fco.gif|left|200px]] |
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| - | '''Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase''' | + | {{Structure |
| + | |PDB= 2fco |SIZE=350|CAPTION= <scene name='initialview01'>2fco</scene>, resolution 1.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] | ||
| + | |GENE= RecU/PrfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 Geobacillus kaustophilus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FCO is a [ | + | 2FCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCO OCA]. |
==Reference== | ==Reference== | ||
| - | Structure, flexibility, and mechanism of the Bacillus stearothermophilus RecU Holliday junction resolvase., Kelly SJ, Li J, Setlow P, Jedrzejas MJ, Proteins. 2007 Sep 1;68(4):961-71. PMID:[http:// | + | Structure, flexibility, and mechanism of the Bacillus stearothermophilus RecU Holliday junction resolvase., Kelly SJ, Li J, Setlow P, Jedrzejas MJ, Proteins. 2007 Sep 1;68(4):961-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17557334 17557334] |
[[Category: Crossover junction endodeoxyribonuclease]] | [[Category: Crossover junction endodeoxyribonuclease]] | ||
[[Category: Geobacillus kaustophilus]] | [[Category: Geobacillus kaustophilus]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:50:49 2008'' |
Revision as of 14:50, 20 March 2008
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| , resolution 1.40Å | |||||||
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| Ligands: | and | ||||||
| Gene: | RecU/PrfA (Geobacillus kaustophilus) | ||||||
| Activity: | Crossover junction endodeoxyribonuclease, with EC number 3.1.22.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase
Overview
Here we report a high resolution structure of RecU-Holliday junction resolvase from Bacillus stearothermophilus. The functional unit of RecU is a homodimer that contains a "mushroom" like structure with a rigid cap and two highly flexible loops extending outwards. These loops appear to be highly flexible/dynamic, and presumably are directly involved in DNA binding and holding it for catalysis. Structural modifications of both the protein and DNA upon their interaction are essential for catalysis. An Mg2+ ion is present in each of the two active sites in this homodimeric enzyme, and two water molecules are coordinated with each Mg2+ ion. Our data are consistent with one of these water molecules acting as a nucleophile and the other as a general acid. The identities of the general base and general acid involved in catalysis and the Lewis acid that stabilizes the pentacovalent transition state phosphate ion are proposed. A model for the RecU-Holliday junction DNA complex is also proposed and discussed in the context of DNA binding and cleavage.
About this Structure
2FCO is a Single protein structure of sequence from Geobacillus kaustophilus. Full crystallographic information is available from OCA.
Reference
Structure, flexibility, and mechanism of the Bacillus stearothermophilus RecU Holliday junction resolvase., Kelly SJ, Li J, Setlow P, Jedrzejas MJ, Proteins. 2007 Sep 1;68(4):961-71. PMID:17557334
Page seeded by OCA on Thu Mar 20 16:50:49 2008
