2fhd
From Proteopedia
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| - | [[Image:2fhd.jpg|left|200px]] | + | [[Image:2fhd.jpg|left|200px]] |
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| - | '''Crystal structure of Crb2 tandem tudor domains''' | + | {{Structure |
| + | |PDB= 2fhd |SIZE=350|CAPTION= <scene name='initialview01'>2fhd</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= rhp9, crb2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Crb2 tandem tudor domains''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FHD is a [ | + | 2FHD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHD OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair., Botuyan MV, Lee J, Ward IM, Kim JE, Thompson JR, Chen J, Mer G, Cell. 2006 Dec 29;127(7):1361-73. PMID:[http:// | + | Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair., Botuyan MV, Lee J, Ward IM, Kim JE, Thompson JR, Chen J, Mer G, Cell. 2006 Dec 29;127(7):1361-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17190600 17190600] |
[[Category: Schizosaccharomyces pombe]] | [[Category: Schizosaccharomyces pombe]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Thompson, J R.]] | [[Category: Thompson, J R.]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
| - | [[Category: tamdem tudor | + | [[Category: tamdem tudor domain]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:52:26 2008'' |
Revision as of 14:52, 20 March 2008
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| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | rhp9, crb2 (Schizosaccharomyces pombe) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Crb2 tandem tudor domains
Overview
Histone lysine methylation has been linked to the recruitment of mammalian DNA repair factor 53BP1 and putative fission yeast homolog Crb2 to DNA double-strand breaks (DSBs), but how histone recognition is achieved has not been established. Here we demonstrate that this link occurs through direct binding of 53BP1 and Crb2 to histone H4. Using X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, we show that, despite low amino acid sequence conservation, both 53BP1 and Crb2 contain tandem tudor domains that interact with histone H4 specifically dimethylated at Lys20 (H4-K20me2). The structure of 53BP1/H4-K20me2 complex uncovers a unique five-residue 53BP1 binding cage, remarkably conserved in the structure of Crb2, that best accommodates a dimethyllysine but excludes a trimethyllysine, thus explaining the methylation state-specific recognition of H4-K20. This study reveals an evolutionarily conserved molecular mechanism of targeting DNA repair proteins to DSBs by direct recognition of H4-K20me2.
About this Structure
2FHD is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.
Reference
Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair., Botuyan MV, Lee J, Ward IM, Kim JE, Thompson JR, Chen J, Mer G, Cell. 2006 Dec 29;127(7):1361-73. PMID:17190600
Page seeded by OCA on Thu Mar 20 16:52:26 2008
