2fk4
From Proteopedia
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| - | [[Image:2fk4.gif|left|200px]] | + | [[Image:2fk4.gif|left|200px]] |
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| - | '''Solution structure of the C-terminal zinc binding domain of the HPV16 E6 oncoprotein''' | + | {{Structure |
| + | |PDB= 2fk4 |SIZE=350|CAPTION= <scene name='initialview01'>2fk4</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Solution structure of the C-terminal zinc binding domain of the HPV16 E6 oncoprotein''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FK4 is a [ | + | 2FK4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_13 Human papillomavirus type 13]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FK4 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis., Nomine Y, Masson M, Charbonnier S, Zanier K, Ristriani T, Deryckere F, Sibler AP, Desplancq D, Atkinson RA, Weiss E, Orfanoudakis G, Kieffer B, Trave G, Mol Cell. 2006 Mar 3;21(5):665-78. PMID:[http:// | + | Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis., Nomine Y, Masson M, Charbonnier S, Zanier K, Ristriani T, Deryckere F, Sibler AP, Desplancq D, Atkinson RA, Weiss E, Orfanoudakis G, Kieffer B, Trave G, Mol Cell. 2006 Mar 3;21(5):665-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16507364 16507364] |
[[Category: Human papillomavirus type 13]] | [[Category: Human papillomavirus type 13]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zinc binding domain]] | [[Category: zinc binding domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:53:21 2008'' |
Revision as of 14:53, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the C-terminal zinc binding domain of the HPV16 E6 oncoprotein
Overview
Oncoprotein E6 is essential for oncogenesis induced by human papillomaviruses (HPVs). The solution structure of HPV16-E6 C-terminal domain reveals a zinc binding fold. A model of full-length E6 is proposed and analyzed in the context of HPV evolution. E6 appears as a chameleon protein combining a conserved structural scaffold with highly variable surfaces participating in generic or specialized HPV functions. We investigated surface residues involved in two specialized activities of high-risk genital HPV E6: p53 tumor suppressor degradation and nucleic acid binding. Screening of E6 surface mutants identified an in vivo p53 degradation-defective mutant that fails to recruit p53 to ubiquitin ligase E6AP and restores high p53 levels in cervical carcinoma cells by competing with endogeneous E6. We also mapped the nucleic acid binding surface of E6, the positive potential of which correlates with genital oncogenicity. E6 structure-function analysis provides new clues for understanding and counteracting the complex pathways of HPV-mediated pathogenesis.
About this Structure
2FK4 is a Single protein structure of sequence from Human papillomavirus type 13. Full crystallographic information is available from OCA.
Reference
Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis., Nomine Y, Masson M, Charbonnier S, Zanier K, Ristriani T, Deryckere F, Sibler AP, Desplancq D, Atkinson RA, Weiss E, Orfanoudakis G, Kieffer B, Trave G, Mol Cell. 2006 Mar 3;21(5):665-78. PMID:16507364
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