2fo1
From Proteopedia
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| - | [[Image:2fo1.gif|left|200px]] | + | [[Image:2fo1.gif|left|200px]] |
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| - | '''Crystal Structure of the CSL-Notch-Mastermind ternary complex bound to DNA''' | + | {{Structure |
| + | |PDB= 2fo1 |SIZE=350|CAPTION= <scene name='initialview01'>2fo1</scene>, resolution 3.12Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= LAG-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]), sel-8, lag-3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]), lin-12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the CSL-Notch-Mastermind ternary complex bound to DNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FO1 is a [ | + | 2FO1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FO1 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the CSL-Notch-Mastermind ternary complex bound to DNA., Wilson JJ, Kovall RA, Cell. 2006 Mar 10;124(5):985-96. PMID:[http:// | + | Crystal structure of the CSL-Notch-Mastermind ternary complex bound to DNA., Wilson JJ, Kovall RA, Cell. 2006 Mar 10;124(5):985-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16530045 16530045] |
[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:54:46 2008'' |
Revision as of 14:54, 20 March 2008
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| , resolution 3.12Å | |||||||
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| Gene: | LAG-1 (Caenorhabditis elegans), sel-8, lag-3 (Caenorhabditis elegans), lin-12 (Caenorhabditis elegans) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the CSL-Notch-Mastermind ternary complex bound to DNA
Overview
Notch signaling mediates communication between cells and is essential for proper embryonic patterning and development. CSL is a DNA binding transcription factor that regulates transcription of Notch target genes by interacting with coregulators. Transcriptional activation requires the displacement of corepressors from CSL by the intracellular portion of the receptor Notch (NotchIC) and the recruitment of the coactivator protein Mastermind to the complex. Here we report the 3.1 A structure of the ternary complex formed by CSL, NotchIC, and Mastermind bound to DNA. As expected, the RAM domain of Notch interacts with the beta trefoil domain of CSL; however, the C-terminal domain of CSL has an unanticipated central role in the interface formed with the Notch ankyrin repeats and Mastermind. Ternary complex formation induces a substantial conformational change within CSL, suggesting a molecular mechanism for the conversion of CSL from a repressor to an activator.
About this Structure
2FO1 is a Protein complex structure of sequences from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Crystal structure of the CSL-Notch-Mastermind ternary complex bound to DNA., Wilson JJ, Kovall RA, Cell. 2006 Mar 10;124(5):985-96. PMID:16530045
Page seeded by OCA on Thu Mar 20 16:54:46 2008
