2fqd
From Proteopedia
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| - | [[Image:2fqd.jpg|left|200px]] | + | [[Image:2fqd.jpg|left|200px]] |
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| - | '''Crystal Structures of E. coli Laccase CueO under different copper binding situations''' | + | {{Structure |
| + | |PDB= 2fqd |SIZE=350|CAPTION= <scene name='initialview01'>2fqd</scene>, resolution 2.400Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene> and <scene name='pdbligand=CIT:CITRIC ACID'>CIT</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structures of E. coli Laccase CueO under different copper binding situations''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FQD is a [ | + | 2FQD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQD OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of E. coli laccase CueO at different copper concentrations., Li X, Wei Z, Zhang M, Peng X, Yu G, Teng M, Gong W, Biochem Biophys Res Commun. 2007 Mar 2;354(1):21-6. Epub 2006 Dec 22. PMID:[http:// | + | Crystal structures of E. coli laccase CueO at different copper concentrations., Li X, Wei Z, Zhang M, Peng X, Yu G, Teng M, Gong W, Biochem Biophys Res Commun. 2007 Mar 2;354(1):21-6. Epub 2006 Dec 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17217912 17217912] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: azurin-like domain]] | [[Category: azurin-like domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:55:35 2008'' |
Revision as of 14:55, 20 March 2008
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| , resolution 2.400Å | |||||||
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| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structures of E. coli Laccase CueO under different copper binding situations
Overview
CueO protein is a hypothetical bacterial laccase and a good laccase candidate for large scale industrial application. Four CueO crystal structures were determined at different copper concentrations. Low copper occupancy in apo-CueO and slow copper reconstitution process in CueO with exogenous copper were demonstrated. These observations well explain the copper dependence of CueO oxidase activity. Structural comparison between CueO and other three fungal laccase proteins indicates that Glu106 in CueO constitutes the primary counter-work for reconstitution of the trinuclear copper site. Mutation of Glu106 to a Phe enhanced CueO oxidation activity and supported this hypothesis. In addition, an extra alpha-helix from Leu351 to Gly378 covers substrate biding pocket of CueO and might compromises the electron transfer from substrate to type I copper.
About this Structure
2FQD is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structures of E. coli laccase CueO at different copper concentrations., Li X, Wei Z, Zhang M, Peng X, Yu G, Teng M, Gong W, Biochem Biophys Res Commun. 2007 Mar 2;354(1):21-6. Epub 2006 Dec 22. PMID:17217912
Page seeded by OCA on Thu Mar 20 16:55:35 2008
Categories: Escherichia coli | Single protein | Gong, W. | Li, X. | Teng, M. | Wei, Z. | Zhang, M. | C2O | CIT | CU | Azurin-like domain
