3pge
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Structure of sumoylated PCNA== | |
| - | === | + | <StructureSection load='3pge' size='340' side='right' caption='[[3pge]], [[Resolution|resolution]] 2.80Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3pge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PGE FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3l0x|3l0x]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pge RCSB], [http://www.ebi.ac.uk/pdbsum/3pge PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Eukaryotic proliferating cell nuclear antigen (PCNA) is a replication accessory protein that functions in DNA replication, repair, and recombination. The various functions of PCNA are regulated by posttranslational modifications including mono-ubiquitylation, which promotes translesion synthesis, and sumoylation, which inhibits recombination. To understand how SUMO modification regulates PCNA, we generated a split SUMO-modified PCNA protein and showed that it supports cell viability and stimulates DNA polymerase delta activity. We then determined its X-ray crystal structure and found that SUMO occupies a position on the back face of the PCNA ring, which is distinct from the position occupied by ubiquitin in the structure of ubiquitin-modified PCNA. We propose that the back of PCNA has evolved to be a site of regulation that can be easily modified without disrupting ongoing reactions on the front of PCNA, such as normal DNA replication. Moreover, these modifications likely allow PCNA to function as a tool belt, whereby proteins can be recruited to the replication machinery via the back of PCNA and be held in reserve until needed. | ||
| - | + | Crystal Structure of SUMO-Modified Proliferating Cell Nuclear Antigen.,Freudenthal BD, Brogie JE, Gakhar L, Kondratick CM, Washington MT J Mol Biol. 2010 Dec 15. PMID:21167178<ref>PMID:21167178</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Proliferating Cell Nuclear Antigen|Proliferating Cell Nuclear Antigen]] | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Brogie, J E | + | [[Category: Brogie, J E]] |
| - | [[Category: Freudenthal, B D | + | [[Category: Freudenthal, B D]] |
| - | [[Category: Gakhar, L | + | [[Category: Gakhar, L]] |
| - | [[Category: Washington, T | + | [[Category: Washington, T]] |
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Dna replication]] | [[Category: Dna replication]] | ||
Revision as of 06:59, 19 December 2014
Structure of sumoylated PCNA
| |||||||||||
