2fqm
From Proteopedia
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| - | [[Image:2fqm.gif|left|200px]] | + | [[Image:2fqm.gif|left|200px]] |
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| - | '''Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus''' | + | {{Structure |
| + | |PDB= 2fqm |SIZE=350|CAPTION= <scene name='initialview01'>2fqm</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FQM is a [ | + | 2FQM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQM OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus., Ding H, Green TJ, Lu S, Luo M, J Virol. 2006 Mar;80(6):2808-14. PMID:[http:// | + | Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus., Ding H, Green TJ, Lu S, Luo M, J Virol. 2006 Mar;80(6):2808-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16501089 16501089] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vesicular stomatitis indiana virus]] | [[Category: Vesicular stomatitis indiana virus]] | ||
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[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:55:38 2008'' |
Revision as of 14:55, 20 March 2008
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| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | P (Vesicular stomatitis Indiana virus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus
Overview
In the replication cycle of nonsegmented negative-strand RNA viruses, the viral RNA-dependent RNA polymerase (L) recognizes a nucleoprotein (N)-enwrapped RNA template during the RNA polymerase reaction. The viral phosphoprotein (P) is a polymerase cofactor essential for this recognition. We report here the 2.3-angstroms-resolution crystal structure of the central domain (residues 107 to 177) of P from vesicular stomatitis virus. The fold of this domain consists of a beta hairpin, an alpha helix, and another beta hairpin. The alpha helix provides the stabilizing force for forming a homodimer, while the two beta hairpins add additional stabilization by forming a four-stranded beta sheet through domain swapping between two molecules. This central dimer positions the N- and C-terminal domains of P to interact with the N and L proteins, allowing the L protein to specifically recognize the nucleocapsid-RNA template and to progress along the template while concomitantly assembling N with nascent RNA. The interdimer interactions observed in the noncrystallographic packing may offer insight into the mechanism of the RNA polymerase processive reaction along the viral nucleocapsid-RNA template.
About this Structure
2FQM is a Single protein structure of sequence from Vesicular stomatitis indiana virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus., Ding H, Green TJ, Lu S, Luo M, J Virol. 2006 Mar;80(6):2808-14. PMID:16501089
Page seeded by OCA on Thu Mar 20 16:55:38 2008
