2fs2

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[[Image:2fs2.gif|left|200px]]<br /><applet load="2fs2" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2fs2.gif|left|200px]]
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caption="2fs2, resolution 2.000&Aring;" />
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'''Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon'''<br />
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{{Structure
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|PDB= 2fs2 |SIZE=350|CAPTION= <scene name='initialview01'>2fs2</scene>, resolution 2.000&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY=
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|GENE= paaI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2FS2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FS2 OCA].
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2FS2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FS2 OCA].
==Reference==
==Reference==
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Structure, function, and mechanism of the phenylacetate pathway hot dog-fold thioesterase PaaI., Song F, Zhuang Z, Finci L, Dunaway-Mariano D, Kniewel R, Buglino JA, Solorzano V, Wu J, Lima CD, J Biol Chem. 2006 Apr 21;281(16):11028-38. Epub 2006 Feb 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16464851 16464851]
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Structure, function, and mechanism of the phenylacetate pathway hot dog-fold thioesterase PaaI., Song F, Zhuang Z, Finci L, Dunaway-Mariano D, Kniewel R, Buglino JA, Solorzano V, Wu J, Lima CD, J Biol Chem. 2006 Apr 21;281(16):11028-38. Epub 2006 Feb 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16464851 16464851]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: protein structure initiative]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: t820]]
[[Category: t820]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:24:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:56:05 2008''

Revision as of 14:56, 20 March 2008

Image:2fs2.gif


PDB ID 2fs2

Drag the structure with the mouse to rotate
, resolution 2.000Å
Ligands:
Gene: paaI (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon


Overview

The structure and biochemical function of the hot dog-fold thioesterase PaaI operative in the aerobic phenylacetate degradation pathway are examined. PaaI showed modest activity with phenylacetyl-coenzyme A, suggestive of a role in coenzyme A release from this pathway intermediate in the event of limiting downstream pathway enzymes. Minimal activity was observed with aliphatic acyl-coenzyme A thioesters, which ruled out PaaI function in the lower phenylacetate pathway. PaaI was most active with ring-hydroxylated phenylacetyl-coenzyme A thioesters. The x-ray crystal structure of the Escherichia coli thioesterase is reported and analyzed to define the structural basis of substrate recognition and catalysis. The contributions of catalytic and substrate binding residues, thus, identified were examined through steady-state kinetic analysis of site-directed mutant proteins.

About this Structure

2FS2 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure, function, and mechanism of the phenylacetate pathway hot dog-fold thioesterase PaaI., Song F, Zhuang Z, Finci L, Dunaway-Mariano D, Kniewel R, Buglino JA, Solorzano V, Wu J, Lima CD, J Biol Chem. 2006 Apr 21;281(16):11028-38. Epub 2006 Feb 7. PMID:16464851

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