2ft0
From Proteopedia
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| - | [[Image:2ft0.gif|left|200px]] | + | [[Image:2ft0.gif|left|200px]] |
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| - | '''Crystal structure of TDP-fucosamine acetyltransferase (WecD)- complex with acetyl-CoA''' | + | {{Structure |
| + | |PDB= 2ft0 |SIZE=350|CAPTION= <scene name='initialview01'>2ft0</scene>, resolution 1.66Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACO:ACETYL COENZYME *A'>ACO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= wecd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of TDP-fucosamine acetyltransferase (WecD)- complex with acetyl-CoA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FT0 is a [ | + | 2FT0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FT0 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis., Hung MN, Rangarajan E, Munger C, Nadeau G, Sulea T, Matte A, J Bacteriol. 2006 Aug;188(15):5606-17. PMID:[http:// | + | Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis., Hung MN, Rangarajan E, Munger C, Nadeau G, Sulea T, Matte A, J Bacteriol. 2006 Aug;188(15):5606-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16855251 16855251] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: gnat fold acetyltransferase]] | [[Category: gnat fold acetyltransferase]] | ||
[[Category: montreal-kingston bacterial structural genomics initiative]] | [[Category: montreal-kingston bacterial structural genomics initiative]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:56:27 2008'' |
Revision as of 14:56, 20 March 2008
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| , resolution 1.66Å | |||||||
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| Ligands: | |||||||
| Gene: | wecd (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of TDP-fucosamine acetyltransferase (WecD)- complex with acetyl-CoA
Overview
Enterobacterial common antigen (ECA) is a polysaccharide found on the outer membrane of virtually all gram-negative enteric bacteria and consists of three sugars, N-acetyl-d-glucosamine, N-acetyl-d-mannosaminuronic acid, and 4-acetamido-4,6-dideoxy-d-galactose, organized into trisaccharide repeating units having the sequence -->3)-alpha-d-Fuc4NAc-(1-->4)-beta-d-ManNAcA-(1-->4)-alpha-d-GlcNAc-(1-->. While the precise function of ECA is unknown, it has been linked to the resistance of Shiga-toxin-producing Escherichia coli (STEC) O157:H7 to organic acids and the resistance of Salmonella enterica to bile salts. The final step in the synthesis of 4-acetamido-4,6-dideoxy-d-galactose, the acetyl-coenzyme A (CoA)-dependent acetylation of the 4-amino group, is carried out by TDP-fucosamine acetyltransferase (WecD). We have determined the crystal structure of WecD in apo form at a 1.95-Angstrom resolution and bound to acetyl-CoA at a 1.66-Angstrom resolution. WecD is a dimeric enzyme, with each monomer adopting the GNAT N-acetyltransferase fold, common to a number of enzymes involved in acetylation of histones, aminoglycoside antibiotics, serotonin, and sugars. The crystal structure of WecD, however, represents the first structure of a GNAT family member that acts on nucleotide sugars. Based on this cocrystal structure, we have used flexible docking to generate a WecD-bound model of the acetyl-CoA-TDP-fucosamine tetrahedral intermediate, representing the structure during acetyl transfer. Our structural data show that WecD does not possess a residue that directly functions as a catalytic base, although Tyr208 is well positioned to function as a general acid by protonating the thiolate anion of coenzyme A.
About this Structure
2FT0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis., Hung MN, Rangarajan E, Munger C, Nadeau G, Sulea T, Matte A, J Bacteriol. 2006 Aug;188(15):5606-17. PMID:16855251
Page seeded by OCA on Thu Mar 20 16:56:27 2008
Categories: Escherichia coli | Single protein | BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative. | Hung, M N. | Matte, A. | Munger, C. | Nadeau, G. | Rangarajan, E. | Sulea, T. | ACO | Bsgi | Gnat fold acetyltransferase | Montreal-kingston bacterial structural genomics initiative | Structural genomic
