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2fv4
From Proteopedia
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| - | [[Image:2fv4.gif|left|200px]] | + | [[Image:2fv4.gif|left|200px]] |
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| - | '''NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain''' | + | {{Structure |
| + | |PDB= 2fv4 |SIZE=350|CAPTION= <scene name='initialview01'>2fv4</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), YMR117C, YM9718.16C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FV4 is a [ | + | 2FV4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain., Wei RR, Schnell JR, Larsen NA, Sorger PK, Chou JJ, Harrison SC, Structure. 2006 Jun;14(6):1003-9. PMID:[http:// | + | Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain., Wei RR, Schnell JR, Larsen NA, Sorger PK, Chou JJ, Harrison SC, Structure. 2006 Jun;14(6):1003-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16765893 16765893] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: complex]] | [[Category: complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:57:11 2008'' |
Revision as of 14:57, 20 March 2008
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| Gene: | YER018C (Saccharomyces cerevisiae), YMR117C, YM9718.16C (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain
Overview
The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an approximately 570 A long, rod-shaped assembly of four proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore.
About this Structure
2FV4 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain., Wei RR, Schnell JR, Larsen NA, Sorger PK, Chou JJ, Harrison SC, Structure. 2006 Jun;14(6):1003-9. PMID:16765893
Page seeded by OCA on Thu Mar 20 16:57:11 2008
