2fy8

From Proteopedia

Revision as of 14:58, 20 March 2008

Crystal structure of MthK rck domain in its ligand-free gating-ring form

Overview

MthK is a prokaryotic Ca(2+)-gated K(+) channel that, like other ligand-gated channels, converts the chemical energy of ligand binding to the mechanical force of channel opening. The channel's eight ligand-binding domains, the RCK domains, form an octameric gating ring in which Ca(2+) binding induces conformational changes that open the channel. Here we present the crystal structures of the MthK gating ring in closed and partially open states at 2.8 A, both obtained from the same crystal grown in the absence of Ca(2+). Furthermore, our biochemical and electrophysiological analyses demonstrate that MthK is regulated by both Ca(2+) and pH. Ca(2+) regulates the channel by changing the equilibrium of the gating ring between closed and open states, while pH regulates channel gating by affecting gating-ring stability. Our findings, along with the previously determined open MthK structure, allow us to elucidate the ligand gating mechanism of RCK-regulated K(+) channels.

About this Structure

2FY8 is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.

Reference

Crystal structures of a ligand-free MthK gating ring: insights into the ligand gating mechanism of K+ channels., Ye S, Li Y, Chen L, Jiang Y, Cell. 2006 Sep 22;126(6):1161-73. PMID:16990139

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