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2g23

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[[Image:2g23.gif|left|200px]]<br /><applet load="2g23" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2g23.gif|left|200px]]
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caption="2g23, resolution 2.30&Aring;" />
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'''The crystal structure of hexameric phenoxazinone synthase'''<br />
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{{Structure
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|PDB= 2g23 |SIZE=350|CAPTION= <scene name='initialview01'>2g23</scene>, resolution 2.30&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY=
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|GENE= phsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1890 Streptomyces antibioticus])
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}}
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'''The crystal structure of hexameric phenoxazinone synthase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2G23 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=C2O:'>C2O</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G23 OCA].
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2G23 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G23 OCA].
==Reference==
==Reference==
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Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center., Smith AW, Camara-Artigas A, Wang M, Allen JP, Francisco WA, Biochemistry. 2006 Apr 11;45(14):4378-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16584173 16584173]
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Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center., Smith AW, Camara-Artigas A, Wang M, Allen JP, Francisco WA, Biochemistry. 2006 Apr 11;45(14):4378-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16584173 16584173]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces antibioticus]]
[[Category: Streptomyces antibioticus]]
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[[Category: multicopper oxidase]]
[[Category: multicopper oxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:27:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:59:33 2008''

Revision as of 14:59, 20 March 2008

Image:2g23.gif


PDB ID 2g23

Drag the structure with the mouse to rotate
, resolution 2.30Å
Ligands: , and
Gene: phsA (Streptomyces antibioticus)
Coordinates: save as pdb, mmCIF, xml



The crystal structure of hexameric phenoxazinone synthase


Overview

The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 A and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 A with a large central cavity that has a diameter of 50 A. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure.

About this Structure

2G23 is a Single protein structure of sequence from Streptomyces antibioticus. Full crystallographic information is available from OCA.

Reference

Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center., Smith AW, Camara-Artigas A, Wang M, Allen JP, Francisco WA, Biochemistry. 2006 Apr 11;45(14):4378-87. PMID:16584173

Page seeded by OCA on Thu Mar 20 16:59:33 2008

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