2g46
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2g46.gif|left|200px]] | + | [[Image:2g46.gif|left|200px]] |
| - | + | ||
| - | '''structure of vSET in complex with meK27 H3 Pept. and cofactor product SAH''' | + | {{Structure |
| + | |PDB= 2g46 |SIZE=350|CAPTION= <scene name='initialview01'>2g46</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1]) | ||
| + | }} | ||
| + | |||
| + | '''structure of vSET in complex with meK27 H3 Pept. and cofactor product SAH''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2G46 is a [ | + | 2G46 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G46 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural insights of the specificity and catalysis of a viral histone H3 lysine 27 methyltransferase., Qian C, Wang X, Manzur K, Sachchidanand, Farooq A, Zeng L, Wang R, Zhou MM, J Mol Biol. 2006 May 26;359(1):86-96. Epub 2006 Mar 20. PMID:[http:// | + | Structural insights of the specificity and catalysis of a viral histone H3 lysine 27 methyltransferase., Qian C, Wang X, Manzur K, Sachchidanand, Farooq A, Zeng L, Wang R, Zhou MM, J Mol Biol. 2006 May 26;359(1):86-96. Epub 2006 Mar 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16603186 16603186] |
[[Category: Paramecium bursaria chlorella virus 1]] | [[Category: Paramecium bursaria chlorella virus 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 30: | ||
[[Category: vset structure]] | [[Category: vset structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:00:09 2008'' |
Revision as of 15:00, 20 March 2008
| |||||||
| Ligands: | |||||||
| Gene: | A612L (Paramecium bursaria Chlorella virus 1) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
structure of vSET in complex with meK27 H3 Pept. and cofactor product SAH
Overview
SET domain lysine methyltransferases are known to catalyze site and state-specific methylation of lysine residues in histones that is fundamental in epigenetic regulation of gene activation and silencing in eukaryotic organisms. Here we report the three-dimensional solution structure of the SET domain histone lysine methyltransferase (vSET) from Paramecium bursaria chlorella virus 1 bound to cofactor S-adenosyl-L-homocysteine and a histone H3 peptide containing mono-methylated lysine 27. The dimeric structure, mimicking an enzyme/cofactor/substrate complex, yields the structural basis of the substrate specificity and methylation multiplicity of the enzyme. Our results from mutagenesis and enzyme kinetics analyses argue that a general base mechanism is less likely for lysine methylation by SET domains; and that the only invariant active site residue tyrosine 105 in vSET facilitates methyl transfer from cofactor to the substrate lysine by aligning intermolecular interactions in the lysine access channel of the enzyme.
About this Structure
2G46 is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.
Reference
Structural insights of the specificity and catalysis of a viral histone H3 lysine 27 methyltransferase., Qian C, Wang X, Manzur K, Sachchidanand, Farooq A, Zeng L, Wang R, Zhou MM, J Mol Biol. 2006 May 26;359(1):86-96. Epub 2006 Mar 20. PMID:16603186
Page seeded by OCA on Thu Mar 20 17:00:09 2008
